TY - JOUR
T1 - Simultaneous spectrophotometric determination of phenolic antioxidant (Bha And Bht) concentrations in pharmaceutical preparations and chewing gums using the H-point standard addition method
AU - Özgür, Mahmure
AU - Kalaycioglu, Zeynep
AU - Dülger, Öznur
PY - 2017
Y1 - 2017
N2 - A simple, rapid, and sensitive spectrophotometric method was developed for the simultaneous de-termination of butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT) concentration in pharmaceutical preparations and chewing gums, without prior separation steps, using the H-point stand-ard addition method (HPSAM).The concentration of one antioxidant was calculated by overlapping spec-tra at two appropriately selected wavelengths at which the interferent, other antioxidant, should has the same absorbance value. Absorbances at two pairs of wavelengths, 265 and 288 nm (with BHA as analyte) or 288 and 293 nm (with BHT as analyte) were monitored, while adding standard solutions of BHA or BHT, respectively. Calibration graphs were determined at 4-20 μgml-1 (r = 0.9981) for BHA and 20-100 μgml-1 (r = 0.9940) for BHT in binary mixtures. The proposed method was tested and validated using various parameters according to ICH guidelines. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 0.48 and 1.51 μgml-1 for BHA and 0.72 and 2.41 μgml-1 for BHT, respectively. The percentage recovery ranges were 100.44-102.50 % for BHA and 96.45-100.04 % for BHT, with relative standard deviations (RSD) less than 1.48 % indicating reasonable repeatability of the method. The intra-day and inter-day precision tests showed reliable RSD values ( < 2 %). The results obtained us-ing HPSAM were statistically compared with results obtained using the derivative spectrophotometric method that was previously reported by us, showing high similarity between results.
AB - A simple, rapid, and sensitive spectrophotometric method was developed for the simultaneous de-termination of butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT) concentration in pharmaceutical preparations and chewing gums, without prior separation steps, using the H-point stand-ard addition method (HPSAM).The concentration of one antioxidant was calculated by overlapping spec-tra at two appropriately selected wavelengths at which the interferent, other antioxidant, should has the same absorbance value. Absorbances at two pairs of wavelengths, 265 and 288 nm (with BHA as analyte) or 288 and 293 nm (with BHT as analyte) were monitored, while adding standard solutions of BHA or BHT, respectively. Calibration graphs were determined at 4-20 μgml-1 (r = 0.9981) for BHA and 20-100 μgml-1 (r = 0.9940) for BHT in binary mixtures. The proposed method was tested and validated using various parameters according to ICH guidelines. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 0.48 and 1.51 μgml-1 for BHA and 0.72 and 2.41 μgml-1 for BHT, respectively. The percentage recovery ranges were 100.44-102.50 % for BHA and 96.45-100.04 % for BHT, with relative standard deviations (RSD) less than 1.48 % indicating reasonable repeatability of the method. The intra-day and inter-day precision tests showed reliable RSD values ( < 2 %). The results obtained us-ing HPSAM were statistically compared with results obtained using the derivative spectrophotometric method that was previously reported by us, showing high similarity between results.
KW - Butylated hydroxyanisole
KW - Butylated hydroxytoluene
KW - Chewing gums
KW - H-point standard addition method
KW - Pharmaceutical preparations
KW - Phenolic antioxidants
KW - Spectrophotometry
UR - http://www.scopus.com/inward/record.url?scp=85039720578&partnerID=8YFLogxK
U2 - 10.20450/mjcce.2017.1330
DO - 10.20450/mjcce.2017.1330
M3 - Article
AN - SCOPUS:85039720578
SN - 1857-5552
VL - 36
SP - 211
EP - 222
JO - Macedonian Journal of Chemistry and Chemical Engineering
JF - Macedonian Journal of Chemistry and Chemical Engineering
IS - 2
ER -