Shtereom i simple windows® based software for stereology. volume and number estimations

Emin Oğuzhan Oğuz, Erdinç Şahin Çonkur, Murat Sari

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)


Stereology has been earlier defined by Wiebel (1970) to be: "a body of mathematical methods relating to three dimensional parameters defining the structure from two dimensional measurements obtainable on sections of the structure. " SHTEREOM I is a simple windows-based software for stereological estimation. In this first part, we describe the implementation of the number and volume estimation tools for unbiased design-based stereology. This software is produced in Visual Basic and can be used on personal computers operated by Microsoft Windows® operating systems that are connected to a conventional camera attached to a microscope and a microcator or a simple dial gauge. Microsoft NET Framework version 1.1 also needs to be downloaded for full use. The features of the SHTEREOM I software are illustrated through examples of stereological estimations in terms of volume and particle numbers for different magnifications (4X-100X). Point-counting grids are available for area estimations and for use with the most efficient volume estimation tool, the Cavalieri technique and are applied to Lizard testicle volume. An unbiased counting frame system is available for number estimations of the objects under investigation, and an on-screen manual stepping module for number estimations through the optical fractionator method is also available for the measurement of increments along the X and Y axes of the microscope stage for the estimation of rat brain hippocampal pyramidal neurons.

Original languageEnglish
Pages (from-to)45-50
Number of pages6
JournalImage Analysis and Stereology
Issue number1
Publication statusPublished - 2007
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2007 Slovenian Society For Stereology And Quantitative Image Analysis.


The authors would like to express their sincere gratitude to Prof. H.J.G. Gundersen and Assoc. Prof. Jens Randel Nyengaard for their useful lectures during their visit to Aarhus University Stereology Department under the EU Leonardo Da Vinci Exchange Programme. They also thank Dr Chris Berrie for Scientific English Language Corrections and Mr. John Hill for some final suggestions. They are also grateful to Assistant Professor Yucel Basimoglu Koca from Adnan Menderes University for providing lizard testis slides.

FundersFunder number
Adnan Menderes Üniversitesi


    • Morphometry
    • Number estimation
    • Stereology
    • Stereology software
    • Volume estimation


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