Abstract
A fully water soluble 3-hydroxyflavone (3HF) derivative, N-(3-hydroxy-4′-flavonyl)-N,N,N-trimethylammonium sulfate (3HFNMe3) was synthesized. Investigation of its emissions at varying wavelengths revealed that it had three emission bands of normal (N∗), anionic (A∗) and tautomeric (T∗), in ultrapure water. Recognition of single-stranded ten ssDNA chains, having different nucleotide sequences was studied, using the ratiometric change of the intensities of the two bands (A∗/T∗), depending upon the varying environment of the 3HFNMe3 with different ssDNA chains. Addition of the ssDNA chains to the 3HFNMe3 solution caused gradual quenching of the A∗ band and had almost no effect on the T∗ band. As the ratios of the two bands (A∗/T∗) vs increasing amount of the ssDNAs generated characteristic curves for each ssDNA chain, it became possible to identify the chains with their characteristic curves.
| Original language | English |
|---|---|
| Pages (from-to) | 391-396 |
| Number of pages | 6 |
| Journal | Journal of Photochemistry and Photobiology B: Biology |
| Volume | 153 |
| DOIs | |
| Publication status | Published - Dec 2015 |
Bibliographical note
Publisher Copyright:© 2015 Elsevier B.V.
Funding
We thank Dr. Andrey Klymchenko of University of Strasbourg for his excellent successions on anionic mechanism, Dr. Mehmet Emin Cinar for useful discussions on computational calculations, and the Scientific and Technological Research Council of Turkey ( 104T081 ), Istanbul Technical University and Unsped Global Lojistik for financial supports. We also thank National Center for High Performance Computing of Turkey (UYBHM) for computing resources.
| Funders | Funder number |
|---|---|
| Istanbul Technical University and Unsped Global Lojistik | |
| Türkiye Bilimsel ve Teknolojik Araştirma Kurumu | 104T081 |
Keywords
- 3-Hydroxyflavone
- DNA
- Fluorophore
- Specific recognition
- Spectroscopic methods