Sensitive fluorescence detection of Ni2+ ions using fluorescein functionalized Fe3O4 nanoparticles

M. T. Shah, A. Balouch, E. Alveroglu*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

53 Citations (Scopus)

Abstract

This study describes a very sensitive fluorescence sensor for the selective nanomolar detection of Ni2+ ions. The Ni2+ ion sensing is based on fluorescence quenching of the fluorophore (fluorescein) in neutral aqueous medium. The fluorescence sensor is composed of a magnetic core and amino silica shell, functionalized with a fluorescein fluorophore. The morphology, physical and chemical properties of the sensing materials were studied by FT-IR spectroscopy, X-ray powder diffraction, vibrating sample magnetometer (VSM) and Transmission Electron Microscopy (TEM). UV-visible and fluorescence spectroscopy were used to characterize the fluorescein functionalized magnetic nanoparticles. The characterization measurements revealed that the fluorescent nanostructures were superparamagnetic in nature with an average particle diameter of 10 nm. The as-fabricated fluorescent nanosensor (Fe3O4@SiO2-NH2-fluorescein) showed an enhanced fluorescence quenching response towards Ni2+ ions in neutral pH medium. The response of the nanosensor was highly selective towards the target species whereas the possible interferences from other metal cations and biological molecules were negligible. The fluorescein probe has a very fast response, it is selective and has a sensitive detection limit (LOD = 0.83 nM) towards Ni2+ ions in neutral medium with a high binding constant (K) value of 3.2 × 104 M-1 for the complex formation between the sensor and Ni2+ ions. These features ensure the potential use of fluorescein functionalized magnetic nanomaterials as a new class of non-toxic biocompatible sensors for biological and environmental applications.

Original languageEnglish
Pages (from-to)1105-1115
Number of pages11
JournalJournal of Materials Chemistry C
Volume6
Issue number5
DOIs
Publication statusPublished - 2018

Bibliographical note

Publisher Copyright:
© 2018 The Royal Society of Chemistry.

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