Selectivity change in the separation of proteins and peptides by capillary electrophoresis using high-molecular-mass polyethyleneimine

Alejandro Cifuentes; Hans Poppe; Johan C. Kraak; F. Bedia Erim

doi:10.1016/0378-4347(96)00004-7

Selectivity change in the separation of proteins and peptides by capillary electrophoresis using high-molecular-mass polyethyleneimine

Alejandro Cifuentes, Hans Poppe, Johan C. Kraak, F. Bedia Erim^*

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

51 Citations (Scopus)

Abstract

A study of the capillary electrophoretic separations of proteins and peptides using high-molecular-mass polyethyleneimine (PEI) is presented. Experiments were performed in the PEI-coated capillaries together with the use of this polymer as a buffer additive under different separation conditions. The effects of pH and the concentration of PEI in the buffer on the electroosmotic flow and the migration orders of biopolymers were investigated. The use of the cationic polymer offers an alternative for the modification of the separation selectivity and resolution of biopolymers.

Original language	English
Pages (from-to)	21-27
Number of pages	7
Journal	Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume	681
Issue number	1
DOIs	https://doi.org/10.1016/0378-4347(96)00004-7
Publication status	Published - 31 May 1996

Funding

A.C. would like to acknowledge the financial support of the European Community (Human Capital and Mobility Programme, bursary No. ERB4OO 1 GT9209 89).

Funders	Funder number
European Community

Keywords

Peptides
Polyethyleneimine
Proteins

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10.1016/0378-4347(96)00004-7

Cite this

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abstract = "A study of the capillary electrophoretic separations of proteins and peptides using high-molecular-mass polyethyleneimine (PEI) is presented. Experiments were performed in the PEI-coated capillaries together with the use of this polymer as a buffer additive under different separation conditions. The effects of pH and the concentration of PEI in the buffer on the electroosmotic flow and the migration orders of biopolymers were investigated. The use of the cationic polymer offers an alternative for the modification of the separation selectivity and resolution of biopolymers.",

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Selectivity change in the separation of proteins and peptides by capillary electrophoresis using high-molecular-mass polyethyleneimine. / Cifuentes, Alejandro; Poppe, Hans; Kraak, Johan C. et al.
In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 681, No. 1, 31.05.1996, p. 21-27.

Research output: Contribution to journal › Article › peer-review

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AU - Cifuentes, Alejandro

AU - Poppe, Hans

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AU - Erim, F. Bedia

PY - 1996/5/31

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Selectivity change in the separation of proteins and peptides by capillary electrophoresis using high-molecular-mass polyethyleneimine

Abstract

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