Rotationally oscillating drill (Ros-Drill©) for mouse ICSI without using mercury

Ali Fuat Ergenc, Ming Wen Li, Mehmet Toner, John D. Biggers, K. C.Kent Lloyd, Nejat Olgac

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)

Abstract

Intracytoplasmic sperm injection (ICSI) is an important assisted reproductive technology (ART). Due to deployment difficulties and low efficiency of the earlier (conventional) version of ICSI, especially in the mouse, a piezo-assisted ICSI technique had evolved as a popular ART methodology in recent years. An important and remaining problem with this technique, however, is that it requires small amounts of mercury to stabilize the pipette tip when piezoelectric force pulses are applied. To eliminate this problem we developed and tested a completely different and mercury-free technology, called the "Ros-Drill

Original languageEnglish
Pages (from-to)1744-1751
Number of pages8
JournalMolecular Reproduction and Development
Volume75
Issue number12
DOIs
Publication statusPublished - Dec 2008
Externally publishedYes

Funding

FundersFunder number
National Center for Research ResourcesR24RR018934

    Keywords

    • Assisted reproduction
    • ICSI
    • Microinjection
    • Micromanipulation

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