Response of the cherry rootstock to water stress induced in vitro

N. Sivritepe; U. Erturk; C. Yerlikaya; I. Turkan; M. Bor; F. Ozdemir

doi:10.1007/s10535-008-0114-4

Response of the cherry rootstock to water stress induced in vitro

N. Sivritepe^*, U. Erturk, C. Yerlikaya, I. Turkan, M. Bor, F. Ozdemir

^*Corresponding author for this work

Department of Chemical Engineering

Research output: Contribution to journal › Article › peer-review

72 Citations (Scopus)

Abstract

The in vitro response of sweet cherry (Prunus cerasus × P. canescens) rootstock Gisela 5 to increasing water deficit in the culture medium was studied. Water stress induced by the incorporation of 1, 2 and 4 % polyethylene glycol (PEG-8000) into the Murashige and Skoog medium was applied for 6 weeks. PEG-induced water stress reduced shoot dry mass, length, water content and relative chlorophyll content. Water stress also induced leaf necrosis without causing loss of viability in the explants. The increase in malondialdehyde content indicated oxidative stress. The activities of antioxidant enzymes, such as superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), peroxidase (POX) and glutathione reductase (GR) were also significantly elevated. The concentrations of K, Ca, Fe and Mn of shoots were decreased.

Original language	English
Pages (from-to)	573-576
Number of pages	4
Journal	Biologia Plantarum
Volume	52
Issue number	3
DOIs	https://doi.org/10.1007/s10535-008-0114-4
Publication status	Published - Sept 2008

Keywords

Antioxidant enzymes
Chlorophyll
Drought injury
Growth
Ion content
Lipid peroxidation

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10.1007/s10535-008-0114-4

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abstract = "The in vitro response of sweet cherry (Prunus cerasus × P. canescens) rootstock Gisela 5 to increasing water deficit in the culture medium was studied. Water stress induced by the incorporation of 1, 2 and 4 % polyethylene glycol (PEG-8000) into the Murashige and Skoog medium was applied for 6 weeks. PEG-induced water stress reduced shoot dry mass, length, water content and relative chlorophyll content. Water stress also induced leaf necrosis without causing loss of viability in the explants. The increase in malondialdehyde content indicated oxidative stress. The activities of antioxidant enzymes, such as superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), peroxidase (POX) and glutathione reductase (GR) were also significantly elevated. The concentrations of K, Ca, Fe and Mn of shoots were decreased.",

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AU - Sivritepe, N.

AU - Erturk, U.

AU - Yerlikaya, C.

AU - Turkan, I.

AU - Bor, M.

AU - Ozdemir, F.

PY - 2008/9

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N2 - The in vitro response of sweet cherry (Prunus cerasus × P. canescens) rootstock Gisela 5 to increasing water deficit in the culture medium was studied. Water stress induced by the incorporation of 1, 2 and 4 % polyethylene glycol (PEG-8000) into the Murashige and Skoog medium was applied for 6 weeks. PEG-induced water stress reduced shoot dry mass, length, water content and relative chlorophyll content. Water stress also induced leaf necrosis without causing loss of viability in the explants. The increase in malondialdehyde content indicated oxidative stress. The activities of antioxidant enzymes, such as superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), peroxidase (POX) and glutathione reductase (GR) were also significantly elevated. The concentrations of K, Ca, Fe and Mn of shoots were decreased.

AB - The in vitro response of sweet cherry (Prunus cerasus × P. canescens) rootstock Gisela 5 to increasing water deficit in the culture medium was studied. Water stress induced by the incorporation of 1, 2 and 4 % polyethylene glycol (PEG-8000) into the Murashige and Skoog medium was applied for 6 weeks. PEG-induced water stress reduced shoot dry mass, length, water content and relative chlorophyll content. Water stress also induced leaf necrosis without causing loss of viability in the explants. The increase in malondialdehyde content indicated oxidative stress. The activities of antioxidant enzymes, such as superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), peroxidase (POX) and glutathione reductase (GR) were also significantly elevated. The concentrations of K, Ca, Fe and Mn of shoots were decreased.

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KW - Chlorophyll

KW - Drought injury

KW - Growth

KW - Ion content

KW - Lipid peroxidation

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Response of the cherry rootstock to water stress induced in vitro

Abstract

Keywords

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