Recognition and modulation of cytochrome c's redox properties using an amphiphilic homopolymer

Britto S. Sandanaraj, Haul Bayraktar, Kothandam Krishnamoorthy, Michael J. Knapp*, S. Thayumanavan

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

An amphiphilic homopolymer scaffold has been used to bind to the protein, cytochrome c. This interaction is analyzed using cyclic voltammetry, native gel electrophoresis, UV-visible absorption, and circular dichroism spectroscopy. The polymer binds to cytochrome c with micromolar affinity and the association of polymer with cytochrome c leads to a structural change of the protein. This conformational change exposes the home unit of the protein, which affords an opportunity to reversibly modulate its electron-transfer properties. We have also shown that the electrostatic binding of polymer to cytochrome c can be used to disrupt its interaction with its natural panner, cytochrome c peroxidase.

Original languageEnglish
Pages (from-to)3891-3897
Number of pages7
JournalLangmuir
Volume23
Issue number7
DOIs
Publication statusPublished - 27 Mar 2007
Externally publishedYes

Funding

FundersFunder number
National Institute of General Medical SciencesR01GM065255

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