Raman tweezers as an alternative diagnostic tool for paroxysmal nocturnal hemoglobinuria

Kaan Batu Soysal, Seyma Parlatan, Metban Mastanzade, Murat Ozbalak, Mustafa Nuri Yenerel, Mehmet Burcin Unlu, Gunay Basar, Ugur Parlatan

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Paroxysmal nocturnal hemoglobinuria (PNH) is a rare disease characterized by hemolysis of red blood cells (RBC) and venous thrombosis. The gold standard method for the diagnosis of this disease is flow cytometry. Here, we propose a combined optical tweezers and Raman spectral (Raman tweezers) approach to analyze blood samples from volunteers with or without PNH conditions. Raman spectroscopy is a well-known method for investigating a material's chemical structure and is also used in molecular analysis of biological compounds. In this study, we trap individual RBCs found in whole blood samples drawn from PNH patients and the control group. Evaluation of the Raman spectra of these cells by band component analysis and machine learning shows a significant difference between the two groups. The specificity and the sensitivity of the training performed by support vector machine (SVM) analysis were found to be 81.8% and 78.3%, respectively. This study shows that an immediate and high accuracy test result is possible for PNH disease by employing Raman tweezers and machine learning.

Original languageEnglish
Pages (from-to)3963-3969
Number of pages7
JournalAnalytical Methods
Volume13
Issue number35
DOIs
Publication statusPublished - 21 Sept 2021

Bibliographical note

Publisher Copyright:
© The Royal Society of Chemistry.

Funding

This study was supported by grants from the Republic of Turkey Ministry of Industry and Technology (Project Number: 2009K120520) and Scientic and Technological Research Council of Turkey (TUBITAK Project Number: 118S113). The authors thank all the volunteers for donating their blood used in this project.

FundersFunder number
Scientic and Technological Research Council of Turkey
TUBITAK118S113

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