TY - JOUR
T1 - Production of structured lipids containing conjugated linolenic acid
T2 - Optimisation by response surface methodology
AU - Elibal, Begum
AU - Suzen, H. Funda
AU - Aksoy, H. Ayse
AU - Ustun, Guldem
AU - Tuter, Melek
PY - 2011/7
Y1 - 2011/7
N2 - Structured lipids containing conjugated linolenic acid (CLNA) were produced separately by enzymatic acidolysis reaction of corn and canola oils (CAO) with bitter gourd (Momordica charantia L) seed oil fatty acids [bitter gourd seed oil fatty acids (BGFA)]. Reactions were conducted using a commercial immobilised sn-1,3-specific lipase from Thermomyces lanuginosa (Lipozyme TL IM) in hexane. The effects of reaction time, substrate molar ratio, temperature and enzyme amount on incorporation yield of CLNA were investigated and optimised by response surface methodology with three-level, two-factor face-centred cube design. When reactions were conducted using 10% enzyme for 3h, the optimum reaction conditions were found for corn oil (CO) as 53.5°C and 5.9:1 BGFA/CO molar ratio. At these conditions, the incorporation of CLNA into CO was determined as 41.4%. However, CLNA incorporation into CAO was resulted as 37% at optimum conditions which were 54.2°C and 6.8:1 BGFA/CAO molar ratio.
AB - Structured lipids containing conjugated linolenic acid (CLNA) were produced separately by enzymatic acidolysis reaction of corn and canola oils (CAO) with bitter gourd (Momordica charantia L) seed oil fatty acids [bitter gourd seed oil fatty acids (BGFA)]. Reactions were conducted using a commercial immobilised sn-1,3-specific lipase from Thermomyces lanuginosa (Lipozyme TL IM) in hexane. The effects of reaction time, substrate molar ratio, temperature and enzyme amount on incorporation yield of CLNA were investigated and optimised by response surface methodology with three-level, two-factor face-centred cube design. When reactions were conducted using 10% enzyme for 3h, the optimum reaction conditions were found for corn oil (CO) as 53.5°C and 5.9:1 BGFA/CO molar ratio. At these conditions, the incorporation of CLNA into CO was determined as 41.4%. However, CLNA incorporation into CAO was resulted as 37% at optimum conditions which were 54.2°C and 6.8:1 BGFA/CAO molar ratio.
KW - Canola oil
KW - Conjugated linolenic acid
KW - Corn oil
KW - Lipozyme TL IM
KW - Response surface methodology
KW - Structured lipid
UR - http://www.scopus.com/inward/record.url?scp=79958769872&partnerID=8YFLogxK
U2 - 10.1111/j.1365-2621.2011.02635.x
DO - 10.1111/j.1365-2621.2011.02635.x
M3 - Article
AN - SCOPUS:79958769872
SN - 0950-5423
VL - 46
SP - 1422
EP - 1427
JO - International Journal of Food Science and Technology
JF - International Journal of Food Science and Technology
IS - 7
ER -