Preparation and characterization of sulfonyl-hydrazine attached poly(styrene-divinylbenzene) beads for separation of albumin

Gulay Bayramoglu; Filiz B. Senkal; Gokce Celik; M. Yakup Arica

doi:10.1016/j.colsurfa.2006.07.043

Preparation and characterization of sulfonyl-hydrazine attached poly(styrene-divinylbenzene) beads for separation of albumin

Gulay Bayramoglu, Filiz B. Senkal, Gokce Celik, M. Yakup Arica^*

^*Corresponding author for this work

Department of Chemistry

Kirikkale University

Research output: Contribution to journal › Article › peer-review

38 Citations (Scopus)

Abstract

Novel sulfonyl-hydrazine carrying poly(styrene-divinylbenzene), poly(S-DVB)-S-NH₂ ion-exchange beads (size between 210 and 425 μm) were prepared via suspension polymerization and, were first used as an ion-exchange support for adsorption of human serum albumin (HSA) from aqueous solution. The influence of pH, equilibrium time, ionic strength and initial albumin concentration on the adsorption capacity of the poly(S-DVB)-S-NH₂ ion-exchange beads has been investigated in a batch system and the unmodified poly(S-DVB) beads were used as control system. Maximum HSA adsorption onto poly(S-DVB)-S-NH₂ ion-exchange beads was found to be 63.05 mg/g at pH 7.0. The experimental equilibrium data for HSA adsorption onto poly(S-DVB)-S-NH₂ beads was well described by the Langmuir isotherm model. Kinetic parameters of this adsorption system were also analyzed for HSA adsorption onto beads and, the first-order rate equations were favorable. Finally, the poly(S-DVB)-S-NH₂ beads was used for the purification of HSA from whole human serum and, the purity of the eluted HSA from the beads was determined as 89% by HPLC from single step purification protocol.

Original language	English
Pages (from-to)	56-63
Number of pages	8
Journal	Colloids and Surfaces A: Physicochemical and Engineering Aspects
Volume	294
Issue number	1-3
DOIs	https://doi.org/10.1016/j.colsurfa.2006.07.043
Publication status	Published - 15 Feb 2007

Keywords

Adsorption
Human serum albumin
Hydrazine
Kinetic parameters
Poly(styrene-divinylbenzene) beads
Suspension polymerization

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10.1016/j.colsurfa.2006.07.043

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title = "Preparation and characterization of sulfonyl-hydrazine attached poly(styrene-divinylbenzene) beads for separation of albumin",

abstract = "Novel sulfonyl-hydrazine carrying poly(styrene-divinylbenzene), poly(S-DVB)-S-NH2 ion-exchange beads (size between 210 and 425 μm) were prepared via suspension polymerization and, were first used as an ion-exchange support for adsorption of human serum albumin (HSA) from aqueous solution. The influence of pH, equilibrium time, ionic strength and initial albumin concentration on the adsorption capacity of the poly(S-DVB)-S-NH2 ion-exchange beads has been investigated in a batch system and the unmodified poly(S-DVB) beads were used as control system. Maximum HSA adsorption onto poly(S-DVB)-S-NH2 ion-exchange beads was found to be 63.05 mg/g at pH 7.0. The experimental equilibrium data for HSA adsorption onto poly(S-DVB)-S-NH2 beads was well described by the Langmuir isotherm model. Kinetic parameters of this adsorption system were also analyzed for HSA adsorption onto beads and, the first-order rate equations were favorable. Finally, the poly(S-DVB)-S-NH2 beads was used for the purification of HSA from whole human serum and, the purity of the eluted HSA from the beads was determined as 89% by HPLC from single step purification protocol.",

keywords = "Adsorption, Human serum albumin, Hydrazine, Kinetic parameters, Poly(styrene-divinylbenzene) beads, Suspension polymerization",

author = "Gulay Bayramoglu and Senkal, {Filiz B.} and Gokce Celik and Arica, {M. Yakup}",

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T1 - Preparation and characterization of sulfonyl-hydrazine attached poly(styrene-divinylbenzene) beads for separation of albumin

AU - Bayramoglu, Gulay

AU - Senkal, Filiz B.

AU - Celik, Gokce

AU - Arica, M. Yakup

PY - 2007/2/15

Y1 - 2007/2/15

N2 - Novel sulfonyl-hydrazine carrying poly(styrene-divinylbenzene), poly(S-DVB)-S-NH2 ion-exchange beads (size between 210 and 425 μm) were prepared via suspension polymerization and, were first used as an ion-exchange support for adsorption of human serum albumin (HSA) from aqueous solution. The influence of pH, equilibrium time, ionic strength and initial albumin concentration on the adsorption capacity of the poly(S-DVB)-S-NH2 ion-exchange beads has been investigated in a batch system and the unmodified poly(S-DVB) beads were used as control system. Maximum HSA adsorption onto poly(S-DVB)-S-NH2 ion-exchange beads was found to be 63.05 mg/g at pH 7.0. The experimental equilibrium data for HSA adsorption onto poly(S-DVB)-S-NH2 beads was well described by the Langmuir isotherm model. Kinetic parameters of this adsorption system were also analyzed for HSA adsorption onto beads and, the first-order rate equations were favorable. Finally, the poly(S-DVB)-S-NH2 beads was used for the purification of HSA from whole human serum and, the purity of the eluted HSA from the beads was determined as 89% by HPLC from single step purification protocol.

AB - Novel sulfonyl-hydrazine carrying poly(styrene-divinylbenzene), poly(S-DVB)-S-NH2 ion-exchange beads (size between 210 and 425 μm) were prepared via suspension polymerization and, were first used as an ion-exchange support for adsorption of human serum albumin (HSA) from aqueous solution. The influence of pH, equilibrium time, ionic strength and initial albumin concentration on the adsorption capacity of the poly(S-DVB)-S-NH2 ion-exchange beads has been investigated in a batch system and the unmodified poly(S-DVB) beads were used as control system. Maximum HSA adsorption onto poly(S-DVB)-S-NH2 ion-exchange beads was found to be 63.05 mg/g at pH 7.0. The experimental equilibrium data for HSA adsorption onto poly(S-DVB)-S-NH2 beads was well described by the Langmuir isotherm model. Kinetic parameters of this adsorption system were also analyzed for HSA adsorption onto beads and, the first-order rate equations were favorable. Finally, the poly(S-DVB)-S-NH2 beads was used for the purification of HSA from whole human serum and, the purity of the eluted HSA from the beads was determined as 89% by HPLC from single step purification protocol.

KW - Adsorption

KW - Human serum albumin

KW - Hydrazine

KW - Kinetic parameters

KW - Poly(styrene-divinylbenzene) beads

KW - Suspension polymerization

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ER -

Preparation and characterization of sulfonyl-hydrazine attached poly(styrene-divinylbenzene) beads for separation of albumin

Abstract

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