Preparation and Characterization of Chitosan/Silver Nano-composite and its Application on Nile Water as Antibacterial Materials

The prepared silver/chitosan nanocomposite and chitosan nanoparticles in this study may demonstrate the potential in optimizing the minimum amount required to achieve complete inactivation of various Coliform bacteria in Nile water. Chitosan nanoparticles were prepared based on the ionic gelation of the prepared chitosan and silver nanoparticles were reduced by Solenostemma Argel extract. Finally, chitosan silver-loaded nanoparticles were prepared by dispersing silver nanoparticles onto the chitosan nanoparticles. The SEM images exhibited a diameter range of 10 nm-30 nm for both of the fabricated silver nanoparticles and chitosan nanoparticles. The UV-Vis analysis confirmed the formation of Ag nanoparticles by the appearance of the characteristic peak at 410 nm. The antibacterial activity of chitosan nanoparticles and silver-loaded nanoparticles was evaluated against the Coliform bacteria. Results show an improvement in the inhibition of the growth of various bacteria tested when silver nanoparticles were introduced which was (0.03g/100ml). Consequently, chitosan silver-loaded nanoparticles could be recommended as an efficient antibacterial material for water disinfection.