Physicochemical and in vitro cytotoxic properties of chitosan from mushroom species (Boletus bovinus and Laccaria laccata)

Alona Oberemko; Asier M. Salaberria; Rita Saule; Gintautas Saulis; Murat Kaya; Jalel Labidi; Vykintas Baublys

doi:10.1016/j.carbpol.2019.05.073

Physicochemical and in vitro cytotoxic properties of chitosan from mushroom species (Boletus bovinus and Laccaria laccata)

Alona Oberemko
, Asier M. Salaberria
, Rita Saule
, Gintautas Saulis
, Murat Kaya
, Jalel Labidi^*
, Vykintas Baublys

^*Corresponding author for this work

Vytautas Magnus University
University of the Basque Country
Aksaray University

Research output: Contribution to journal › Article › peer-review

47 Citations (Scopus)

Abstract

Chitosan samples from two mushroom species (Boletus bovinus, Laccaria laccata)were obtained and characterized by viscosimetry, attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR), elemental analyses (EA), nuclear magnetic resonance spectroscopy (¹³C NMR), X-ray diffraction (XRD)and thermogravimetric (TGA)analyses. Properties of the fungal chitosan samples were compared to commercial low-molecular weight chitosan, crustacean chitosan (Cervimunida johni)and chitosan obtained from an insect (Hilobius abietis). Additionally, the cytotoxic properties of chitosan in vitro on cancerous hepatoma and non-cancerous ovary cells cultivated on films with different chitosan concentrations was evaluated. As a conclusion, this study clearly revealed that low-molecular weight chitosan films and solutions with high degree of deacetylation can act cytotoxically on both tumor MH-22A and normal CHO cells in vitro. Consequently, this work may be useful for further investigations of natural anticancer products in medical areas.

Original language	English
Pages (from-to)	1-9
Number of pages	9
Journal	Carbohydrate Polymers
Volume	221
DOIs	https://doi.org/10.1016/j.carbpol.2019.05.073
Publication status	Published - 1 Oct 2019
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2019 Elsevier Ltd

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Cancer
Chitosan
Deacetylation
Low-Molecular weight
Mushroom

Access to Document

10.1016/j.carbpol.2019.05.073

Cite this

@article{43598649e3f547368bf39e43d0cd3307,

title = "Physicochemical and in vitro cytotoxic properties of chitosan from mushroom species (Boletus bovinus and Laccaria laccata)",

abstract = "Chitosan samples from two mushroom species (Boletus bovinus, Laccaria laccata)were obtained and characterized by viscosimetry, attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR), elemental analyses (EA), nuclear magnetic resonance spectroscopy (13C NMR), X-ray diffraction (XRD)and thermogravimetric (TGA)analyses. Properties of the fungal chitosan samples were compared to commercial low-molecular weight chitosan, crustacean chitosan (Cervimunida johni)and chitosan obtained from an insect (Hilobius abietis). Additionally, the cytotoxic properties of chitosan in vitro on cancerous hepatoma and non-cancerous ovary cells cultivated on films with different chitosan concentrations was evaluated. As a conclusion, this study clearly revealed that low-molecular weight chitosan films and solutions with high degree of deacetylation can act cytotoxically on both tumor MH-22A and normal CHO cells in vitro. Consequently, this work may be useful for further investigations of natural anticancer products in medical areas.",

keywords = "Cancer, Chitosan, Deacetylation, Low-Molecular weight, Mushroom",

author = "Alona Oberemko and Salaberria, \{Asier M.\} and Rita Saule and Gintautas Saulis and Murat Kaya and Jalel Labidi and Vykintas Baublys",

note = "Publisher Copyright: {\textcopyright} 2019 Elsevier Ltd",

year = "2019",

month = oct,

day = "1",

doi = "10.1016/j.carbpol.2019.05.073",

language = "English",

volume = "221",

pages = "1--9",

journal = "Carbohydrate Polymers",

issn = "0144-8617",

publisher = "Elsevier Ltd",

}

TY - JOUR

T1 - Physicochemical and in vitro cytotoxic properties of chitosan from mushroom species (Boletus bovinus and Laccaria laccata)

AU - Oberemko, Alona

AU - Salaberria, Asier M.

AU - Saule, Rita

AU - Saulis, Gintautas

AU - Kaya, Murat

AU - Labidi, Jalel

AU - Baublys, Vykintas

PY - 2019/10/1

Y1 - 2019/10/1

N2 - Chitosan samples from two mushroom species (Boletus bovinus, Laccaria laccata)were obtained and characterized by viscosimetry, attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR), elemental analyses (EA), nuclear magnetic resonance spectroscopy (13C NMR), X-ray diffraction (XRD)and thermogravimetric (TGA)analyses. Properties of the fungal chitosan samples were compared to commercial low-molecular weight chitosan, crustacean chitosan (Cervimunida johni)and chitosan obtained from an insect (Hilobius abietis). Additionally, the cytotoxic properties of chitosan in vitro on cancerous hepatoma and non-cancerous ovary cells cultivated on films with different chitosan concentrations was evaluated. As a conclusion, this study clearly revealed that low-molecular weight chitosan films and solutions with high degree of deacetylation can act cytotoxically on both tumor MH-22A and normal CHO cells in vitro. Consequently, this work may be useful for further investigations of natural anticancer products in medical areas.

AB - Chitosan samples from two mushroom species (Boletus bovinus, Laccaria laccata)were obtained and characterized by viscosimetry, attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR), elemental analyses (EA), nuclear magnetic resonance spectroscopy (13C NMR), X-ray diffraction (XRD)and thermogravimetric (TGA)analyses. Properties of the fungal chitosan samples were compared to commercial low-molecular weight chitosan, crustacean chitosan (Cervimunida johni)and chitosan obtained from an insect (Hilobius abietis). Additionally, the cytotoxic properties of chitosan in vitro on cancerous hepatoma and non-cancerous ovary cells cultivated on films with different chitosan concentrations was evaluated. As a conclusion, this study clearly revealed that low-molecular weight chitosan films and solutions with high degree of deacetylation can act cytotoxically on both tumor MH-22A and normal CHO cells in vitro. Consequently, this work may be useful for further investigations of natural anticancer products in medical areas.

KW - Cancer

KW - Chitosan

KW - Deacetylation

KW - Low-Molecular weight

KW - Mushroom

UR - https://www.scopus.com/pages/publications/85066263062

U2 - 10.1016/j.carbpol.2019.05.073

DO - 10.1016/j.carbpol.2019.05.073

M3 - Article

C2 - 31227147

AN - SCOPUS:85066263062

SN - 0144-8617

VL - 221

SP - 1

EP - 9

JO - Carbohydrate Polymers

JF - Carbohydrate Polymers

ER -

Physicochemical and in vitro cytotoxic properties of chitosan from mushroom species (Boletus bovinus and Laccaria laccata)

Abstract

Bibliographical note

UN SDGs

Keywords

Access to Document

Other files and links

Fingerprint

Cite this