TY - JOUR
T1 - Physicochemical and in vitro cytotoxic properties of chitosan from mushroom species (Boletus bovinus and Laccaria laccata)
AU - Oberemko, Alona
AU - Salaberria, Asier M.
AU - Saule, Rita
AU - Saulis, Gintautas
AU - Kaya, Murat
AU - Labidi, Jalel
AU - Baublys, Vykintas
N1 - Publisher Copyright:
© 2019 Elsevier Ltd
PY - 2019/10/1
Y1 - 2019/10/1
N2 - Chitosan samples from two mushroom species (Boletus bovinus, Laccaria laccata)were obtained and characterized by viscosimetry, attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR), elemental analyses (EA), nuclear magnetic resonance spectroscopy (13C NMR), X-ray diffraction (XRD)and thermogravimetric (TGA)analyses. Properties of the fungal chitosan samples were compared to commercial low-molecular weight chitosan, crustacean chitosan (Cervimunida johni)and chitosan obtained from an insect (Hilobius abietis). Additionally, the cytotoxic properties of chitosan in vitro on cancerous hepatoma and non-cancerous ovary cells cultivated on films with different chitosan concentrations was evaluated. As a conclusion, this study clearly revealed that low-molecular weight chitosan films and solutions with high degree of deacetylation can act cytotoxically on both tumor MH-22A and normal CHO cells in vitro. Consequently, this work may be useful for further investigations of natural anticancer products in medical areas.
AB - Chitosan samples from two mushroom species (Boletus bovinus, Laccaria laccata)were obtained and characterized by viscosimetry, attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR), elemental analyses (EA), nuclear magnetic resonance spectroscopy (13C NMR), X-ray diffraction (XRD)and thermogravimetric (TGA)analyses. Properties of the fungal chitosan samples were compared to commercial low-molecular weight chitosan, crustacean chitosan (Cervimunida johni)and chitosan obtained from an insect (Hilobius abietis). Additionally, the cytotoxic properties of chitosan in vitro on cancerous hepatoma and non-cancerous ovary cells cultivated on films with different chitosan concentrations was evaluated. As a conclusion, this study clearly revealed that low-molecular weight chitosan films and solutions with high degree of deacetylation can act cytotoxically on both tumor MH-22A and normal CHO cells in vitro. Consequently, this work may be useful for further investigations of natural anticancer products in medical areas.
KW - Cancer
KW - Chitosan
KW - Deacetylation
KW - Low-Molecular weight
KW - Mushroom
UR - http://www.scopus.com/inward/record.url?scp=85066263062&partnerID=8YFLogxK
U2 - 10.1016/j.carbpol.2019.05.073
DO - 10.1016/j.carbpol.2019.05.073
M3 - Article
C2 - 31227147
AN - SCOPUS:85066263062
SN - 0144-8617
VL - 221
SP - 1
EP - 9
JO - Carbohydrate Polymers
JF - Carbohydrate Polymers
ER -
