Abstract
A simple approach for the preparation of a clickable poly(ethylene glycol)-based hydrogel as a polymeric support for protein immobilization via photoinitiated free radical polymerization of poly(ethylene glycol) diacrylate and propargyl acrylate is established. Bioconjugation to the obtained gels was achieved by azide-alkyne click reaction with azido-functionalized anti-immunoglobulin G (anti-IgG) and anti-His tag antibodies. Evaluation of the affinities of the PEG hydrogels to the corresponding substrates (IgG and His-tagged YFP, respectively) indicates their specific binding capability.
| Original language | English |
|---|---|
| Pages (from-to) | 129-136 |
| Number of pages | 8 |
| Journal | Designed Monomers and Polymers |
| Volume | 18 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 17 Feb 2015 |
Bibliographical note
Publisher Copyright:© 2014 Taylor & Francis.
Funding
The authors are thankful for Istanbul Technical University Research Fund and TUBITAK (The Scientific and Technical Research Council of Turkey) Project No. 113Z234. M.C. thanks TUBITAK for the financial support by means of a graduate program.
| Funders | Funder number |
|---|---|
| Istanbul Technical University Research Fund | |
| TUBITAK | |
| Türkiye Bilimsel ve Teknolojik Araştirma Kurumu | 113Z234 |
Keywords
- bioconjugation
- click chemistry
- hydrogels
- photopolymerization
- protein adsorption