Photoinduced in situ formation of clickable PEG hydrogels and their antibody conjugation

Muhammet U. Kahveci; Mustafa Ciftci; Serap Evran; Suna Timur; Yusuf Yagci

doi:10.1080/15685551.2014.971392

Photoinduced in situ formation of clickable PEG hydrogels and their antibody conjugation

Muhammet U. Kahveci, Mustafa Ciftci, Serap Evran, Suna Timur, Yusuf Yagci^*

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

A simple approach for the preparation of a clickable poly(ethylene glycol)-based hydrogel as a polymeric support for protein immobilization via photoinitiated free radical polymerization of poly(ethylene glycol) diacrylate and propargyl acrylate is established. Bioconjugation to the obtained gels was achieved by azide-alkyne click reaction with azido-functionalized anti-immunoglobulin G (anti-IgG) and anti-His tag antibodies. Evaluation of the affinities of the PEG hydrogels to the corresponding substrates (IgG and His-tagged YFP, respectively) indicates their specific binding capability.

Original language	English
Pages (from-to)	129-136
Number of pages	8
Journal	Designed Monomers and Polymers
Volume	18
Issue number	2
DOIs	https://doi.org/10.1080/15685551.2014.971392
Publication status	Published - 17 Feb 2015

Bibliographical note

Publisher Copyright:
© 2014 Taylor & Francis.

Keywords

bioconjugation
click chemistry
hydrogels
photopolymerization
protein adsorption

Access to Document

10.1080/15685551.2014.971392

Cite this

@article{98368e168d8345a5b536c6787f064d20,

title = "Photoinduced in situ formation of clickable PEG hydrogels and their antibody conjugation",

abstract = "A simple approach for the preparation of a clickable poly(ethylene glycol)-based hydrogel as a polymeric support for protein immobilization via photoinitiated free radical polymerization of poly(ethylene glycol) diacrylate and propargyl acrylate is established. Bioconjugation to the obtained gels was achieved by azide-alkyne click reaction with azido-functionalized anti-immunoglobulin G (anti-IgG) and anti-His tag antibodies. Evaluation of the affinities of the PEG hydrogels to the corresponding substrates (IgG and His-tagged YFP, respectively) indicates their specific binding capability.",

keywords = "bioconjugation, click chemistry, hydrogels, photopolymerization, protein adsorption",

author = "Kahveci, {Muhammet U.} and Mustafa Ciftci and Serap Evran and Suna Timur and Yusuf Yagci",

note = "Publisher Copyright: {\textcopyright} 2014 Taylor & Francis.",

year = "2015",

month = feb,

day = "17",

doi = "10.1080/15685551.2014.971392",

language = "English",

volume = "18",

pages = "129--136",

journal = "Designed Monomers and Polymers",

issn = "1385-772X",

publisher = "Taylor and Francis Ltd.",

number = "2",

}

TY - JOUR

T1 - Photoinduced in situ formation of clickable PEG hydrogels and their antibody conjugation

AU - Kahveci, Muhammet U.

AU - Ciftci, Mustafa

AU - Evran, Serap

AU - Timur, Suna

AU - Yagci, Yusuf

PY - 2015/2/17

Y1 - 2015/2/17

N2 - A simple approach for the preparation of a clickable poly(ethylene glycol)-based hydrogel as a polymeric support for protein immobilization via photoinitiated free radical polymerization of poly(ethylene glycol) diacrylate and propargyl acrylate is established. Bioconjugation to the obtained gels was achieved by azide-alkyne click reaction with azido-functionalized anti-immunoglobulin G (anti-IgG) and anti-His tag antibodies. Evaluation of the affinities of the PEG hydrogels to the corresponding substrates (IgG and His-tagged YFP, respectively) indicates their specific binding capability.

AB - A simple approach for the preparation of a clickable poly(ethylene glycol)-based hydrogel as a polymeric support for protein immobilization via photoinitiated free radical polymerization of poly(ethylene glycol) diacrylate and propargyl acrylate is established. Bioconjugation to the obtained gels was achieved by azide-alkyne click reaction with azido-functionalized anti-immunoglobulin G (anti-IgG) and anti-His tag antibodies. Evaluation of the affinities of the PEG hydrogels to the corresponding substrates (IgG and His-tagged YFP, respectively) indicates their specific binding capability.

KW - bioconjugation

KW - click chemistry

KW - hydrogels

KW - photopolymerization

KW - protein adsorption

UR - http://www.scopus.com/inward/record.url?scp=84916210923&partnerID=8YFLogxK

U2 - 10.1080/15685551.2014.971392

DO - 10.1080/15685551.2014.971392

M3 - Article

AN - SCOPUS:84916210923

SN - 1385-772X

VL - 18

SP - 129

EP - 136

JO - Designed Monomers and Polymers

JF - Designed Monomers and Polymers

IS - 2

ER -

Photoinduced in situ formation of clickable PEG hydrogels and their antibody conjugation

Abstract

Bibliographical note

Keywords

Access to Document

Other files and links

Fingerprint

Cite this