Photo-crosslinked PVA/PEI electrospun nanofiber membranes: Preparation and preliminary evaluation in virus clearance tests

Bihter Zeytuncu; Melike Ürper; İsmail Koyuncu; Volodymyr V. Tarabara

doi:10.1016/j.seppur.2018.01.002

Photo-crosslinked PVA/PEI electrospun nanofiber membranes: Preparation and preliminary evaluation in virus clearance tests

Bihter Zeytuncu^*, Melike Ürper, İsmail Koyuncu, Volodymyr V. Tarabara

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

24 Citations (Scopus)

Abstract

We report on the preparation of electropositive nanofiber membranes by electrospinning with in situ photo-crosslinking and their preliminary evaluation in virus adsorption and removal tests. Poly(vinyl alcohol) (PVA) and polyethyleneimine (PEI) were modified with glycidyl methacrylate, to form an acrylated crosslinked polymer (a-PVA/a-PEI) upon UV exposure during the electrospinning process. The a-PVA/a-PEI nanofibers were electrospun on a non-woven polyester support to form an electropositive (ζ = 7 mV at pH 7.4) and hydrophilic (θ_w∼53^°) membrane with the mean pore size of 0.48 μm. The microfilter had the specific permeate flux of ∼6.9 · 10⁴ L/(m²·h·bar), comparable with that of commercially available membranes of similar nominal pore sizes. Adsorption of the negatively charged and hydrophilic bacteriophage MS2 (d∼27 nm) onto the membrane followed Freundlich isotherm and could be classified as favorable with the average adsorption intensity n^-1∼0.91. The 99% retention of MS2 in flow-through virus clearance tests was attributed to adsorption and was likely controlled by the limited detention time within the membrane.

Original language	English
Pages (from-to)	432-438
Number of pages	7
Journal	Separation and Purification Technology
Volume	197
DOIs	https://doi.org/10.1016/j.seppur.2018.01.002
Publication status	Published - 31 May 2018

Bibliographical note

Publisher Copyright:
© 2018 Elsevier B.V.

Funding

This material is based upon work supported in part by The Scientific and Technological Research Council of Turkey (TUBITAK) BIDEB-2219 Postdoctoral Research Scholarship Program under grant number 1059B191401279 to Dr. Bihter Zeytuncu and in part by the National Science Foundation Partnerships for International Education and Research Program under Grant IIA-1243433 . The authors thank Ms. Abigail Vanderberg and Ms. Amy Albin (Center for Advanced Microscopy, MSU) for their assistance with electron microscopy imaging. We are grateful to Dr. Merlin L. Bruening (Department of Chemistry, MSU) for providing access to the FTIR spectrometer and Dr. Scott Calabrese Barton (Department of Chemical Engineering and Materials Science, MSU) for letting us use the electrospinning system in his laboratory. We also thank two anonymous reviewers for their insightful criticism and suggestions. Appendix A

Funders	Funder number
National Science Foundation Partnerships for International Education and Research Program	IIA-1243433
Scientific and Technological Research Council of Turkey
TUBITAK	1059B191401279

Keywords

Bacteriophage MS2
Electrospinning
Microfiltration
Nanofiber membranes
Photo-crosslinking

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10.1016/j.seppur.2018.01.002

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title = "Photo-crosslinked PVA/PEI electrospun nanofiber membranes: Preparation and preliminary evaluation in virus clearance tests",

abstract = "We report on the preparation of electropositive nanofiber membranes by electrospinning with in situ photo-crosslinking and their preliminary evaluation in virus adsorption and removal tests. Poly(vinyl alcohol) (PVA) and polyethyleneimine (PEI) were modified with glycidyl methacrylate, to form an acrylated crosslinked polymer (a-PVA/a-PEI) upon UV exposure during the electrospinning process. The a-PVA/a-PEI nanofibers were electrospun on a non-woven polyester support to form an electropositive (ζ = 7 mV at pH 7.4) and hydrophilic (θw∼53°) membrane with the mean pore size of 0.48 μm. The microfilter had the specific permeate flux of ∼6.9 · 104 L/(m2·h·bar), comparable with that of commercially available membranes of similar nominal pore sizes. Adsorption of the negatively charged and hydrophilic bacteriophage MS2 (d∼27 nm) onto the membrane followed Freundlich isotherm and could be classified as favorable with the average adsorption intensity n-1∼0.91. The 99% retention of MS2 in flow-through virus clearance tests was attributed to adsorption and was likely controlled by the limited detention time within the membrane.",

keywords = "Bacteriophage MS2, Electrospinning, Microfiltration, Nanofiber membranes, Photo-crosslinking",

author = "Bihter Zeytuncu and Melike {\"U}rper and İsmail Koyuncu and Tarabara, {Volodymyr V.}",

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AU - Tarabara, Volodymyr V.

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AB - We report on the preparation of electropositive nanofiber membranes by electrospinning with in situ photo-crosslinking and their preliminary evaluation in virus adsorption and removal tests. Poly(vinyl alcohol) (PVA) and polyethyleneimine (PEI) were modified with glycidyl methacrylate, to form an acrylated crosslinked polymer (a-PVA/a-PEI) upon UV exposure during the electrospinning process. The a-PVA/a-PEI nanofibers were electrospun on a non-woven polyester support to form an electropositive (ζ = 7 mV at pH 7.4) and hydrophilic (θw∼53°) membrane with the mean pore size of 0.48 μm. The microfilter had the specific permeate flux of ∼6.9 · 104 L/(m2·h·bar), comparable with that of commercially available membranes of similar nominal pore sizes. Adsorption of the negatively charged and hydrophilic bacteriophage MS2 (d∼27 nm) onto the membrane followed Freundlich isotherm and could be classified as favorable with the average adsorption intensity n-1∼0.91. The 99% retention of MS2 in flow-through virus clearance tests was attributed to adsorption and was likely controlled by the limited detention time within the membrane.

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Photo-crosslinked PVA/PEI electrospun nanofiber membranes: Preparation and preliminary evaluation in virus clearance tests

Abstract

Bibliographical note

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Keywords

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