Performance and microbial community variations in thermophilic anaerobic digesters treating OTC medicated cow manure under different operational conditions

Çağri Akyol; Gokhan Turker; Orhan Ince; Emine Ertekin; Oya Üstüner; Bahar Ince

doi:10.1016/j.biortech.2016.01.071

Performance and microbial community variations in thermophilic anaerobic digesters treating OTC medicated cow manure under different operational conditions

Çağri Akyol^*, Gokhan Turker, Orhan Ince, Emine Ertekin, Oya Üstüner, Bahar Ince

^*Corresponding author for this work

Department of Environmental Engineering

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

This study aimed to determine the fate and effect of oxytetracycline (OTC) and its metabolites during thermophilic anaerobic digestion of cow manure. OTC-medicated and non-medicated digesters were operated at 55 °C with different volatile solids (VS) concentrations (4% and 6%) and mixing rates (90 and 120 rpm). OTC and its metabolites were measured by HPLC and LC/MS/MS, respectively. Microbial community dynamics were monitored by denaturing gradient gel electrophoresis (DGGE) and real-time PCR (qPCR). Approximately 2. mg/L initial OTC concentration caused 10-30% inhibition on biogas production and higher inhibition was observed as mixing rate increased. DGGE results indicated that OTC caused a shift in bacterial community structure and several species became dominant with time. Archaeal community decreased throughout the digestion period. RNA based qPCR analyses showed that gene copy numbers of bacteria and Methanomicrobiales declined in all digesters whereas gene copy numbers of Methanobacteriales and Methanosarcinales increased in high mixing rate digesters.

Original language	English
Pages (from-to)	191-198
Number of pages	8
Journal	Bioresource Technology
Volume	205
DOIs	https://doi.org/10.1016/j.biortech.2016.01.071
Publication status	Published - 1 Apr 2016

Bibliographical note

Publisher Copyright:
© 2016 Elsevier Ltd.

Funding

This study was supported by the Scientific and Technical Research Council of Turkey (TUBITAK, Project No: 109Y275 ) and Boğaziçi University Research Fund (Project No: 11Y00P4).

Funders	Funder number
TUBITAK	109Y275
Türkiye Bilimsel ve Teknolojik Araştirma Kurumu
Boğaziçi Üniversitesi	11Y00P4

Keywords

Cow manure
DGGE
Oxytetracycline
Real-time PCR
Thermophilic anaerobic digestion

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10.1016/j.biortech.2016.01.071

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abstract = "This study aimed to determine the fate and effect of oxytetracycline (OTC) and its metabolites during thermophilic anaerobic digestion of cow manure. OTC-medicated and non-medicated digesters were operated at 55 °C with different volatile solids (VS) concentrations (4% and 6%) and mixing rates (90 and 120 rpm). OTC and its metabolites were measured by HPLC and LC/MS/MS, respectively. Microbial community dynamics were monitored by denaturing gradient gel electrophoresis (DGGE) and real-time PCR (qPCR). Approximately 2. mg/L initial OTC concentration caused 10-30% inhibition on biogas production and higher inhibition was observed as mixing rate increased. DGGE results indicated that OTC caused a shift in bacterial community structure and several species became dominant with time. Archaeal community decreased throughout the digestion period. RNA based qPCR analyses showed that gene copy numbers of bacteria and Methanomicrobiales declined in all digesters whereas gene copy numbers of Methanobacteriales and Methanosarcinales increased in high mixing rate digesters.",

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AU - Turker, Gokhan

AU - Ince, Orhan

AU - Ertekin, Emine

AU - Üstüner, Oya

AU - Ince, Bahar

PY - 2016/4/1

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Performance and microbial community variations in thermophilic anaerobic digesters treating OTC medicated cow manure under different operational conditions

Abstract

Bibliographical note

Funding

Keywords

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