Pectin–Zeolite-Based Wound Dressings with Controlled Albumin Release

Banu Kocaaga, Ozge Kurkcuoglu, Melkon Tatlier, Gizem Dinler-Doganay, Saime Batirel, Fatma Seniha Güner*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

Hypoalbuminemia can lead to poor and delayed wound healing, while it is also associated with acute myocardial infarction, heart failure, malignancies, and COVID-19. In elective surgery, patients with low albumin have high risks of postoperative wound complications. Here, we propose a novel cost-effective wound dressing material based on low-methoxy pectin and NaA-zeolite particles with controlled albumin release properties. We focused on both albumin adsorption and release phenomena for wounds with excess exudate. Firstly, we investigated albumin dynamics and calculated electrostatic surfaces at experimental pH values in water by using molecular dynamics methods. Then, we studied in detail pectin–zeolite hydrogels with both adsorption and diffusion into membrane methods using different pH values and albumin concentrations. To understand if uploaded albumin molecules preserved their secondary conformation in different formulations, we monitored the effect of pH and albumin concentration on the conformational changes in albumin after it was released from the hydrogels by using CD-UV spectroscopy analyses. Our results indicate that at pH 6.4, BSA-containing films preserved the protein’s folded structure while the protein was being released to the external buffer solutions. In vitro wound healing assay indicated that albumin-loaded hydrogels showed no toxic effects on the fibroblast cells.

Original languageEnglish
Article number460
JournalPolymers
Volume14
Issue number3
DOIs
Publication statusPublished - 1 Feb 2022

Bibliographical note

Publisher Copyright:
© 2022 by the authors. Licensee MDPI, Basel, Switzerland.

Funding

Funding: This research was funded by The Istanbul Technical University Scientific Research Projects (BAP) with project number MGA-2018-41165.

FundersFunder number
Istanbul Teknik ÜniversitesiMGA-2018-41165

    Keywords

    • Albumin
    • Pectin
    • Wound dressing
    • Zeolite

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