Optimization of enzymatic methanolysis of soybean oil by response surface methodology

Seda Demirkol, H. Wyse Aksoy*, Melek Tüter, Guldem Ustun, Dursun Ali Sasmaz

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)

Abstract

Enzymatic methanolysis of refined soybean oil with methanol was investigated using Rhizomucor miehei lipase, Lipozyme RM IM, in n-hexane for reaction times of 30 min. Response surface methodology (RSM) based on three-level, three-factor (variable) face-centered cube design was used for the optimization of methanolysis. The independent variables that affect the methanolysis reaction conducted in n-hexane are temperature (°C), enzyme/oil weight ratio, and oil/methanol molar ratio. A good quadratic model was obtained for the methyl ester production by multiple regression and backward elimination. A linear relationship was observed between the observed and predicted values (R2 = 0.9635). The effects of temperature and enzyme amount, which affected methyl ester content of the product (response) positively, were significant (P < 0.01). The quadratic term of temperature and the interaction term of enzyme amount with temperature affected the response negatively (P < 0.01). The interaction term of enzyme amount with substrate mole ratio had a positive effect on the response (P < 0.05). Critical conditions for the response at which methyl ester content of the product was 76.9% were determined to be 50°C, 2.37 methanol/oil mole ratio, and 0.09 enzyme/oil weight ratio.

Original languageEnglish
Pages (from-to)929-932
Number of pages4
JournalJAOCS, Journal of the American Oil Chemists' Society
Volume83
Issue number11
DOIs
Publication statusPublished - Nov 2006

Keywords

  • Alcoholysis
  • Biodiesel
  • Lipase
  • Optimization
  • Response surface methodology
  • Rhizomucor miehei
  • Soybean oil

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