Optimization of diclofenac quantification from wastewater treatment plant sludge by ultrasonication assisted extraction

Emel Topuz, Sevgi Sari, Gamze Ozdemir, Egemen Aydin, Elif Pehlivanoglu-Mantas, Didem Okutman Tas*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)


A rapid quantification method of diclofenac from sludge samples through ultrasonication assisted extraction and solid phase extraction (SPE) was developed and used for the quantification of diclofenac concentrations in sludge samples with liquid chromatography/tandem mass spectrometry (LC-MS/MS). Although the concentration of diclofenac in sludge samples taken from different units of wastewater treatment plants in Istanbul was below the limit of quantification (LOQ; 5. ng/g), an optimized method for sludge samples along with the total mass balances in a wastewater treatment plant can be used to determine the phase with which diclofenac is mostly associated. Hence, the results will provide information on fate and transport of diclofenac, as well as on the necessity of alternative removal processes. In addition, since the optimization procedure is provided in detail, it is possible for other researchers to use this procedure as a starting point for the determination of other emerging pollutants in wastewater sludge samples.

Original languageEnglish
Pages (from-to)48-54
Number of pages7
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Publication statusPublished - 1 May 2014


This work was funded by The Scientific and Technological Research Council of Turkey (TUBITAK project # 110Y319) and ITU BAP Project # 36258.

FundersFunder number
ITU BAP36258
Türkiye Bilimsel ve Teknolojik Araştirma Kurumu


    • LC-MS/MS
    • Method
    • Micropollutants
    • Pharmaceutical
    • Solid phase extraction
    • Ultrasonication


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