Novel electrochemical biosensor for Escherichia coli using gold-coated tungsten wires and antibody functionalized short multiwalled carbon nanotubes

Tahsin Ertaş*, Bircan Dinç, Recep Üstünsoy, Hülya Eraslan, Ali Fuat Ergenç, Muhammet Bektaş

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Rapid and easy detection of pathogens, especially bacteria, plays an important role in daily life in the face of increasing environmental pollution. Nanosensors focus on carbon nanotubes, which can bind various molecules to large surfaces and have high electron conductivity. They are widely used in the development of electrochemical biosensors. In this study, a new design of electrochemical biosensors is presented by integrating gold-coated tungsten wires (GC-TW) and acid-functionalized multi-walled carbon nanotubes (MWCNTs) in a two-step coating process. GC-TWs are coated with acid-functionalized short MWCNTs. Fourier transforms infrared spectroscopy (FTIR), transmission electron microscopy (TEM), and scanning electron microscopy (SEM) results show efficient immobilization of the electrodes. The performance for Escherichia coli (E. coli) K-12 is evaluated based upon the change in current following antibody-antigen interactions. Bioimpedance measurements are performed from 50 Hz to 10 MHz to achieve optimal coverage of impedance changes. The measurements show that the electrode has a detection limit of 0.8 CFU/mL. The response of the electrode saturates when the bacterial concentration is increased to 1.70 x 102 CFU/mL. The biosensor is capable of detecting E. coli between 0.8 and 1.70 × 102 CFU/mL. The data show that this MWCNT-based biosensor can be developed into a highly sensitive device for E. coli.

Original languageEnglish
Pages (from-to)109-124
Number of pages16
JournalInstrumentation Science and Technology
Volume52
Issue number2
DOIs
Publication statusPublished - 2024

Bibliographical note

Publisher Copyright:
© 2023 Taylor & Francis Group, LLC.

Funding

This work was supported by Istanbul University Scientific Research Projects Unit by Project Number 37475. We would also like to thank the Bahcesehir University, Faculty of Medicine, and İstanbul Technical University, Department of Control and Automation Engineering, for their laboratory facilities and support.

FundersFunder number
Istanbul Üniversitesi37475
Bahçeşehir Üniversitesi

    Keywords

    • electrochemical biosensor
    • electrochemical impedance
    • Escherichia coli
    • multiwalled carbon nanotubes (MWCNTs)

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