Newly isolated sporopollenin microcages from Cedrus libani and Pinus nigra as carrier for Oxaliplatin; xCELLigence RTCA-based release assay

Muhammad Mujtaba; Bahar Akyuz Yilmaz; Demet Cansaran-Duman; Lalehan Akyuz; Sevcan Yangın; Murat Kaya; Talip Çeter; Khalid Mahmood Khawar

doi:10.1007/s00289-020-03531-7

Newly isolated sporopollenin microcages from Cedrus libani and Pinus nigra as carrier for Oxaliplatin; xCELLigence RTCA-based release assay

Muhammad Mujtaba^*, Bahar Akyuz Yilmaz, Demet Cansaran-Duman, Lalehan Akyuz, Sevcan Yangın, Murat Kaya, Talip Çeter, Khalid Mahmood Khawar

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Sporopollenin-mediated control drug delivery has been studied extensively owing to its desirable physicochemical and biological properties. Herein, sporopollenin was successfully extracted from C. libani and P. nigra pollens followed by loading of a commonly known anticancer drug Oxaliplatin. Drug loading and physicochemical features were confirmed by using light microscopy, FT-IR, SEM and TGA. For the first-time, real-time cell analyzer system xCELLigence was employed to record the Oxaliplatin loaded sporopollenin-mediated cell death (CaCo-2 and Vero cells) in real time. Both the release assays confirmed the slow release of oxaliplatin from sporopollenin for around 40–45 h. The expression of MYC and FOXO-3 genes has been significantly increased in CaCo2 cell and decreased non-cancerous Vero cell confirming the fact that sporopollenin-mediated control release of oxaliplatin is promoting apoptosis cell death preventing the spread of negative effects on nearby healthy cells. All the results suggested that C. libani and P. nigra can be suitable candidates for the slow delivery of drugs.

Original language	English
Pages (from-to)	519-540
Number of pages	22
Journal	Polymer Bulletin
Volume	79
Issue number	1
DOIs	https://doi.org/10.1007/s00289-020-03531-7
Publication status	Published - Jan 2022
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2021, The Author(s), under exclusive licence to Springer-Verlag GmbH, DE part of Springer Nature.

Funding

MM would like to thanks the scientific and technological council of Turkey (TÜBİTAK) for supporting his thesis under program number TÜBİTAK-2215. The authors would like to acknowledge the technical and human support provided by Biotechnology Institute, Ankara University, and Scientific and Technological Application and Research Center, Aksaray University, Turkey. MM would like to thanks the scientific and technological council of Turkey (T?B?TAK) for supporting his thesis under program number T?B?TAK-2215. The authors would like to acknowledge the technical and human support provided by Biotechnology Institute, Ankara University, and Scientific and Technological Application and Research Center, Aksaray University, Turkey.

Funders	Funder number
Ankara University, and Scientific and Technological Application and Research Center
Ankara Universitesi Biyoteknoloji Enstitusu
Türkiye Bilimsel ve Teknolojik Araştirma Kurumu	TÜBİTAK-2215
Aksaray Üniversitesi
Scientific and Technological Council of Turkey

Keywords

Controlled-drug release
Real-time cell analyzer
Sporopollenin
xCELLigence

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1007/s00289-020-03531-7

Cite this

@article{9d65ce3420d94dd1b5afb038ca173206,

title = "Newly isolated sporopollenin microcages from Cedrus libani and Pinus nigra as carrier for Oxaliplatin; xCELLigence RTCA-based release assay",

abstract = "Sporopollenin-mediated control drug delivery has been studied extensively owing to its desirable physicochemical and biological properties. Herein, sporopollenin was successfully extracted from C. libani and P. nigra pollens followed by loading of a commonly known anticancer drug Oxaliplatin. Drug loading and physicochemical features were confirmed by using light microscopy, FT-IR, SEM and TGA. For the first-time, real-time cell analyzer system xCELLigence was employed to record the Oxaliplatin loaded sporopollenin-mediated cell death (CaCo-2 and Vero cells) in real time. Both the release assays confirmed the slow release of oxaliplatin from sporopollenin for around 40–45 h. The expression of MYC and FOXO-3 genes has been significantly increased in CaCo2 cell and decreased non-cancerous Vero cell confirming the fact that sporopollenin-mediated control release of oxaliplatin is promoting apoptosis cell death preventing the spread of negative effects on nearby healthy cells. All the results suggested that C. libani and P. nigra can be suitable candidates for the slow delivery of drugs.",

keywords = "Controlled-drug release, Real-time cell analyzer, Sporopollenin, xCELLigence",

author = "Muhammad Mujtaba and Yilmaz, {Bahar Akyuz} and Demet Cansaran-Duman and Lalehan Akyuz and Sevcan Yangın and Murat Kaya and Talip {\c C}eter and Khawar, {Khalid Mahmood}",

note = "Publisher Copyright: {\textcopyright} 2021, The Author(s), under exclusive licence to Springer-Verlag GmbH, DE part of Springer Nature.",

year = "2022",

month = jan,

doi = "10.1007/s00289-020-03531-7",

language = "English",

volume = "79",

pages = "519--540",

journal = "Polymer Bulletin",

issn = "0170-0839",

publisher = "Springer Verlag",

number = "1",

}

TY - JOUR

T1 - Newly isolated sporopollenin microcages from Cedrus libani and Pinus nigra as carrier for Oxaliplatin; xCELLigence RTCA-based release assay

AU - Mujtaba, Muhammad

AU - Yilmaz, Bahar Akyuz

AU - Cansaran-Duman, Demet

AU - Akyuz, Lalehan

AU - Yangın, Sevcan

AU - Kaya, Murat

AU - Çeter, Talip

AU - Khawar, Khalid Mahmood

PY - 2022/1

Y1 - 2022/1

N2 - Sporopollenin-mediated control drug delivery has been studied extensively owing to its desirable physicochemical and biological properties. Herein, sporopollenin was successfully extracted from C. libani and P. nigra pollens followed by loading of a commonly known anticancer drug Oxaliplatin. Drug loading and physicochemical features were confirmed by using light microscopy, FT-IR, SEM and TGA. For the first-time, real-time cell analyzer system xCELLigence was employed to record the Oxaliplatin loaded sporopollenin-mediated cell death (CaCo-2 and Vero cells) in real time. Both the release assays confirmed the slow release of oxaliplatin from sporopollenin for around 40–45 h. The expression of MYC and FOXO-3 genes has been significantly increased in CaCo2 cell and decreased non-cancerous Vero cell confirming the fact that sporopollenin-mediated control release of oxaliplatin is promoting apoptosis cell death preventing the spread of negative effects on nearby healthy cells. All the results suggested that C. libani and P. nigra can be suitable candidates for the slow delivery of drugs.

AB - Sporopollenin-mediated control drug delivery has been studied extensively owing to its desirable physicochemical and biological properties. Herein, sporopollenin was successfully extracted from C. libani and P. nigra pollens followed by loading of a commonly known anticancer drug Oxaliplatin. Drug loading and physicochemical features were confirmed by using light microscopy, FT-IR, SEM and TGA. For the first-time, real-time cell analyzer system xCELLigence was employed to record the Oxaliplatin loaded sporopollenin-mediated cell death (CaCo-2 and Vero cells) in real time. Both the release assays confirmed the slow release of oxaliplatin from sporopollenin for around 40–45 h. The expression of MYC and FOXO-3 genes has been significantly increased in CaCo2 cell and decreased non-cancerous Vero cell confirming the fact that sporopollenin-mediated control release of oxaliplatin is promoting apoptosis cell death preventing the spread of negative effects on nearby healthy cells. All the results suggested that C. libani and P. nigra can be suitable candidates for the slow delivery of drugs.

KW - Controlled-drug release

KW - Real-time cell analyzer

KW - Sporopollenin

KW - xCELLigence

UR - http://www.scopus.com/inward/record.url?scp=85098644215&partnerID=8YFLogxK

U2 - 10.1007/s00289-020-03531-7

DO - 10.1007/s00289-020-03531-7

M3 - Article

AN - SCOPUS:85098644215

SN - 0170-0839

VL - 79

SP - 519

EP - 540

JO - Polymer Bulletin

JF - Polymer Bulletin

IS - 1

ER -

Newly isolated sporopollenin microcages from Cedrus libani and Pinus nigra as carrier for Oxaliplatin; xCELLigence RTCA-based release assay

Abstract

Bibliographical note

Funding

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this