Nanoclays for lipase immobilization: Biocatalyst characterization and activity in polyester synthesis

Hale öztürk; Eric Pollet; Vincent Phalip; Yüksel Güvenilir; Luc Avérous

doi:10.3390/polym8120416

Nanoclays for lipase immobilization: Biocatalyst characterization and activity in polyester synthesis

Hale öztürk, Eric Pollet^*, Vincent Phalip, Yüksel Güvenilir, Luc Avérous

^*Corresponding author for this work

Department of Chemical Engineering

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

The immobilization of Candida antarctica lipase B (CALB) was performed by physical adsorption on both neat and organo-modified forms of sepiolite and montmorillonite. The influence of different parameters, e.g., solvent, enzyme loading, cross-linking, and type of clay support, on immobilization efficiency and catalyst hydrolytic activity has been investigated. The highest hydrolytic activities were obtained for CALB immobilized on organo-modified clay minerals, highlighting the beneficial effect of organo-modification. The esterification activity of these CALB/organoclay catalysts was also tested in the ring-opening polymerization of "-caprolactone. The polymerization kinetics observed for clay-immobilized catalysts confirmed that CALB adsorbed on organo-modified montmorillonite (CALB/MMTMOD) was the highest-performing catalytic system.

Original language	English
Article number	149
Journal	Polymers
Volume	8
Issue number	12
DOIs	https://doi.org/10.3390/polym8120416
Publication status	Published - 2016

Bibliographical note

Publisher Copyright:
© 2016 by the authors.

Keywords

Candida antarctica lipase B
Caprolactone polymerization
Immobilization
Montmorillonite
Organoclay
Sepiolite

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10.3390/polym8120416

Cite this

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title = "Nanoclays for lipase immobilization: Biocatalyst characterization and activity in polyester synthesis",

abstract = "The immobilization of Candida antarctica lipase B (CALB) was performed by physical adsorption on both neat and organo-modified forms of sepiolite and montmorillonite. The influence of different parameters, e.g., solvent, enzyme loading, cross-linking, and type of clay support, on immobilization efficiency and catalyst hydrolytic activity has been investigated. The highest hydrolytic activities were obtained for CALB immobilized on organo-modified clay minerals, highlighting the beneficial effect of organo-modification. The esterification activity of these CALB/organoclay catalysts was also tested in the ring-opening polymerization of {"}-caprolactone. The polymerization kinetics observed for clay-immobilized catalysts confirmed that CALB adsorbed on organo-modified montmorillonite (CALB/MMTMOD) was the highest-performing catalytic system.",

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author = "Hale {\"o}zt{\"u}rk and Eric Pollet and Vincent Phalip and Y{\"u}ksel G{\"u}venilir and Luc Av{\'e}rous",

note = "Publisher Copyright: {\textcopyright} 2016 by the authors.",

year = "2016",

doi = "10.3390/polym8120416",

language = "English",

volume = "8",

journal = "Polymers",

issn = "2073-4360",

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TY - JOUR

T1 - Nanoclays for lipase immobilization

T2 - Biocatalyst characterization and activity in polyester synthesis

AU - öztürk, Hale

AU - Pollet, Eric

AU - Phalip, Vincent

AU - Güvenilir, Yüksel

AU - Avérous, Luc

PY - 2016

Y1 - 2016

N2 - The immobilization of Candida antarctica lipase B (CALB) was performed by physical adsorption on both neat and organo-modified forms of sepiolite and montmorillonite. The influence of different parameters, e.g., solvent, enzyme loading, cross-linking, and type of clay support, on immobilization efficiency and catalyst hydrolytic activity has been investigated. The highest hydrolytic activities were obtained for CALB immobilized on organo-modified clay minerals, highlighting the beneficial effect of organo-modification. The esterification activity of these CALB/organoclay catalysts was also tested in the ring-opening polymerization of "-caprolactone. The polymerization kinetics observed for clay-immobilized catalysts confirmed that CALB adsorbed on organo-modified montmorillonite (CALB/MMTMOD) was the highest-performing catalytic system.

AB - The immobilization of Candida antarctica lipase B (CALB) was performed by physical adsorption on both neat and organo-modified forms of sepiolite and montmorillonite. The influence of different parameters, e.g., solvent, enzyme loading, cross-linking, and type of clay support, on immobilization efficiency and catalyst hydrolytic activity has been investigated. The highest hydrolytic activities were obtained for CALB immobilized on organo-modified clay minerals, highlighting the beneficial effect of organo-modification. The esterification activity of these CALB/organoclay catalysts was also tested in the ring-opening polymerization of "-caprolactone. The polymerization kinetics observed for clay-immobilized catalysts confirmed that CALB adsorbed on organo-modified montmorillonite (CALB/MMTMOD) was the highest-performing catalytic system.

KW - Candida antarctica lipase B

KW - Caprolactone polymerization

KW - Immobilization

KW - Montmorillonite

KW - Organoclay

KW - Sepiolite

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DO - 10.3390/polym8120416

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VL - 8

JO - Polymers

JF - Polymers

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M1 - 149

ER -

Nanoclays for lipase immobilization: Biocatalyst characterization and activity in polyester synthesis

Abstract

Bibliographical note

Keywords

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