Monitoring of spectroscopic changes of a single trapped fission yeast cell by using a Raman tweezers set-up

G. Başar*, S. Kin

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

We demonstrate an improvement of the sensitivity of a Raman tweezers set-up, which combines optical tweezers with Raman spectroscopy. The system was tested by taking the Raman spectrum of a 4.6 μm diameter polystyrene sphere trapped in an aqueous solution. The improvement of sensitivity of the set-up was achieved by adjusting the trap depth for maximum signal to noise ratio (SNR). The maximum SNR was obtained by investigating the Raman peak of a trapped polystyrene sphere at 1001 cm-1 according to trap depth. With this system, a single trapped living Schizosaccharomyces Pombe yeast cell was sensitively monitored by taking the kinetic Raman spectra for more than 2 h. The relative intensity decrease in amide I and amide III bands, frequency increase in amide I band together with alterations in tyrosine marker band around 850 cm-1 was observed, which indicates alterations in the hydration state of protein by time progressing.

Original languageEnglish
Pages (from-to)4998-5003
Number of pages6
JournalOptics Communications
Volume281
Issue number19
DOIs
Publication statusPublished - 1 Oct 2008

Keywords

  • Optical trapping
  • Raman spectroscopy

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