Abstract
We demonstrate an improvement of the sensitivity of a Raman tweezers set-up, which combines optical tweezers with Raman spectroscopy. The system was tested by taking the Raman spectrum of a 4.6 μm diameter polystyrene sphere trapped in an aqueous solution. The improvement of sensitivity of the set-up was achieved by adjusting the trap depth for maximum signal to noise ratio (SNR). The maximum SNR was obtained by investigating the Raman peak of a trapped polystyrene sphere at 1001 cm-1 according to trap depth. With this system, a single trapped living Schizosaccharomyces Pombe yeast cell was sensitively monitored by taking the kinetic Raman spectra for more than 2 h. The relative intensity decrease in amide I and amide III bands, frequency increase in amide I band together with alterations in tyrosine marker band around 850 cm-1 was observed, which indicates alterations in the hydration state of protein by time progressing.
Original language | English |
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Pages (from-to) | 4998-5003 |
Number of pages | 6 |
Journal | Optics Communications |
Volume | 281 |
Issue number | 19 |
DOIs | |
Publication status | Published - 1 Oct 2008 |
Keywords
- Optical trapping
- Raman spectroscopy