Microbial endogenous response to acute inhibitory impact of antibiotics

I. Pala-Ozkok*, G. Kor-Bicakci, E. U. Çokgör, D. Jonas, D. Orhon

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Enhanced endogenous respiration was observed as the significant/main response of the aerobic microbial culture under pulse exposure to antibiotics: sulfamethoxazole, tetracycline and erythromycin. Peptone mixture and acetate were selected as organic substrates to compare the effect of complex and simple substrates. Experiments were conducted with microbial cultures acclimated to different sludge ages of 10 and 2 days, to visualize the effect of culture history. Evaluation relied on modeling of oxygen uptake rate profiles, reflecting the effect of all biochemical reactions associated with substrate utilization. Model calibration exhibited significant increase in values of endogenous respiration rate coefficient with all antibiotic doses. Enhancement of endogenous respiration was different with antibiotic type and initial dose. Results showed that both peptone mixture and acetate cultures harbored resistance genes against the tested antibiotics, which suggests that biomass spends cellular maintenance energy for activating the required antibiotic resistance mechanisms to survive, supporting higher endogenous decay rates. Abbreviations: (Formula presented.) : maximum growth rate for XH (day−1); KS: half saturation constant for growth of XH (mg COD/L); bH: endogenous decay rate for XH (day−1); kh: maximum hydrolysis rate for SH1 (day−1); KX: hydrolysis half saturation constant for SH1(mg COD/L); khx: maximum hydrolysis rate for XS1 (day−1); KXX: hydrolysis half saturation constant for XS1 (mg COD/L); kSTO: maximum storage rate of PHA by XH (day−1); (Formula presented.) : maximum growth rate on PHA for XH (day−1); KSTO: half saturation constant for storage of PHA by XH (mg COD/L); XH1: initial active biomass (mg COD/L).

Original languageEnglish
Pages (from-to)1626-1637
Number of pages12
JournalEnvironmental Technology (United Kingdom)
Volume39
Issue number13
DOIs
Publication statusPublished - 3 Jul 2018

Bibliographical note

Publisher Copyright:
© 2017 Informa UK Limited, trading as Taylor & Francis Group.

Funding

This study was conducted with the support of the Turkish Academy of Sciences (Türkiye Bilimler Akademisi) – Fellowship Program for Integrated Doctoral Studies, Istanbul Technical University (Istanbul Teknik Üniversitesi) – Scientific Research Fund (Project Nr: 33742, 33680).

FundersFunder number
Istanbul Teknik Üniversitesi) – Scientific Research Fund
Türkiye Bilimler Akademisi
Istanbul Teknik Üniversitesi33742, 33680

    Keywords

    • Antibiotics
    • activated sludge modeling
    • acute impact
    • enhanced endogenous respiration
    • respirometry

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