Abstract
Auxotrophic mutants may have physiological alterations and sensitivities which are not generally recognized. Such features are shown here by observations that final cell densities attained by several leucine-auxotrophic Saccharomyces cerevisiae strains depend differently on the initial leucine concentration in the medium. Furthermore, complementing such auxotrophic strains with the plasmid-based LEU2 selection marker resulted in different final cell densities than chromosomal expression of LEU2 in the otherwise isogenic, prototrophic strains. These results warn that auxotrophic host-related physiological influences overlay any metabolic effect of a cloned gene expressed in such a host, clearly complicating interpretation of the effect of that gene's product in scientific or metabolic engineering research.
| Original language | English |
|---|---|
| Pages (from-to) | 611-616 |
| Number of pages | 6 |
| Journal | Biotechnology Letters |
| Volume | 21 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - 1999 |
| Externally published | Yes |
Funding
This work was supported by the Swiss Bunde-samt für Bildung und Wissenschaft (BBW) within the Framework IV Biotechnology Programme of the European Commission.
| Funders | Funder number |
|---|---|
| BBW | |
| European Commission | |
| Bayerisches Staatsministerium für Bildung und Kultus, Wissenschaft und Kunst |
Keywords
- Auxotrophy
- Growth physiology
- Leucine
- Metabolic engineering
- Saccharomyces cerevisiae