Mechanism of nonpolar model substances to inhibit primary gushing induced by hydrophobin HFBI

Zahra Shokribousjein*, David Riveros Galan, Patricia Losada-Pérez, Patrick Wagner, Jeroen Lammertyn, Iulia Arghir, Asefeh Golreihan, Hubert Verachtert, Ahmet Alper Aydin, Marc De Maeyer, Jean Titze, Vladimír Ilberg, Guy Derdelinckx

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


In this work, the interactions of a well-studied hydrophobin with different types of nonpolar model substances and their impact on primary gushing is evaluated. The nature, length, and degree of saturation of nonpolar molecules are key parameters defining the gushing ability or inhibition. When mixed with hydrophobins, the nonpolar molecule-hydrophobin assembly acts as a less gushing or no gushing system. This effect can be explained in the framework of a competition effect between non-polar systems and CO2 to interact with the hydrophobic patch of the hydrophobin. Interactions of these molecules with hydrophobins are promoted as a result of the similar size of the nonpolar molecules with the hydrophobic patch of the protein, at the expense of the formation of nanobubbles with CO2. In order to prove the presence of interactions and to unravel the mechanisms behind them, a complete set of experimental techniques was used. Surface sensitive techniques clearly show the presence of the interactions, whose nature is not covalent nor hydrogen bonding according to infrared spectroscopy results. Interactions were also reflected by particle size analysis in which mixtures of particles displayed larger size than their pure component counterparts. Upon mixing with nonpolar molecules, the gushing ability of the protein is significantly disrupted.

Original languageEnglish
Pages (from-to)4673-4682
Number of pages10
JournalJournal of Agricultural and Food Chemistry
Issue number18
Publication statusPublished - 13 May 2015

Bibliographical note

Publisher Copyright:
© 2015 American Chemical Society.


  • gushing
  • hydrocarbons
  • hydrophobins
  • infrared spectroscopy
  • particle size analysis
  • quartz crystal microbalance with dissipation
  • self-assembly
  • surface plasmon resonance


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