TY - GEN
T1 - Maximum imaging depth comparison in porcine vocal folds using 776-nm vs. 1552-nm excitation wavelengths
AU - Yildirim, Murat
AU - Ferhanoglu, Onur
AU - Kobler, James B.
AU - Zeitels, Steven M.
AU - Ben-Yakar, Adela
PY - 2013
Y1 - 2013
N2 - Vocal fold scarring is one of the major causes of voice disorders and may arise from overuse or post-surgical wound healing. One promising treatment utilizes the injection of soft biomaterials aimed at restoring viscoelasticity of the outermost vibratory layer of the vocal fold, superficial lamina propria (SLP). However, the density of the tissue and the required injection pressure impair proper localization of the injected biomaterial in SLP. To enhance treatment effectiveness, we are investigating a technique to image and ablate sub-epithelial planar voids in vocal folds using ultrafast laser pulses to better localize the injected biomaterial. It is challenging to optimize the excitation wavelength to perform imaging and ablation at depths suitable for clinical use. Here, we compare maximum imaging depth using two photon autofluorescence and second harmonic generation with third-harmonic generation imaging modalities for healthy porcine vocal folds. We used a home-built inverted nonlinear scanning microscope together with a high repetition rate (2 MHz) ultrafast fiber laser (Raydiance Inc.). We acquired both two-photon autofluorescence and second harmonic generation signals using 776 nm wavelength and third harmonic generation signals using 1552 nm excitation wavelength. We observed that maximum imaging depth with 776 nm wavelength is significantly improved from 114 μm to 205 μm when third harmonic generation is employed using 1552 nm wavelength, without any observable damage in the tissue.
AB - Vocal fold scarring is one of the major causes of voice disorders and may arise from overuse or post-surgical wound healing. One promising treatment utilizes the injection of soft biomaterials aimed at restoring viscoelasticity of the outermost vibratory layer of the vocal fold, superficial lamina propria (SLP). However, the density of the tissue and the required injection pressure impair proper localization of the injected biomaterial in SLP. To enhance treatment effectiveness, we are investigating a technique to image and ablate sub-epithelial planar voids in vocal folds using ultrafast laser pulses to better localize the injected biomaterial. It is challenging to optimize the excitation wavelength to perform imaging and ablation at depths suitable for clinical use. Here, we compare maximum imaging depth using two photon autofluorescence and second harmonic generation with third-harmonic generation imaging modalities for healthy porcine vocal folds. We used a home-built inverted nonlinear scanning microscope together with a high repetition rate (2 MHz) ultrafast fiber laser (Raydiance Inc.). We acquired both two-photon autofluorescence and second harmonic generation signals using 776 nm wavelength and third harmonic generation signals using 1552 nm excitation wavelength. We observed that maximum imaging depth with 776 nm wavelength is significantly improved from 114 μm to 205 μm when third harmonic generation is employed using 1552 nm wavelength, without any observable damage in the tissue.
KW - Healthy porcine vocal folds
KW - Maximum imaging depth
KW - Second-harmonic generation
KW - Third-harmonic generation
KW - Two-photon autofluorescence
KW - Ultrafast fiber laser
KW - Vocal fold scarring
UR - http://www.scopus.com/inward/record.url?scp=84878687623&partnerID=8YFLogxK
U2 - 10.1117/12.2005630
DO - 10.1117/12.2005630
M3 - Conference contribution
AN - SCOPUS:84878687623
SN - 9780819493576
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Multiphoton Microscopy in the Biomedical Sciences XIII
T2 - Multiphoton Microscopy in the Biomedical Sciences XIII
Y2 - 3 February 2013 through 5 February 2013
ER -