Long-circulating Janus nanoparticles made by electrohydrodynamic co-jetting for systemic drug delivery applications

Sahar Rahmani, Carlos H. Villa, Acacia F. Dishman, Marika E. Grabowski, Daniel C. Pan, Hakan Durmaz, Asish C. Misra, Laura Colón-Meléndez, Michael J. Solomon, Vladimir R. Muzykantov, Joerg Lahann*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

29 Citations (Scopus)

Abstract

Background: Nanoparticles with controlled physical properties have been widely used for controlled release applications. In addition to shape, the anisotropic nature of the particles can be an important design criterion to ensure selective surface modification or independent release of combinations of drugs.Purpose: Electrohydrodynamic (EHD) co-jetting is used for the fabrication of uniform anisotropic nanoparticles with individual compartments and initial physicochemical and biological characterization is reported.Methods: EHD co-jetting is used to create nanoparticles, which are characterized at each stage with scanning electron microscopy (SEM), structured illumination microscopy (SIM), dynamic light scattering (DLS) and nanoparticle tracking analysis (NTA). Surface immobilization techniques are used to incorporate polyethylene glycol (PEG) and I125 radiolabels into the nanoparticles. Particles are injected in mice and the particle distribution after 1, 4 and 24 hours is assessed.Results and discussion: Nanoparticles with an average diameter of 105.7 nm are prepared by EHD co-jetting. The particles contain functional chemical groups for further surface modification and radiolabeling. The density of PEG molecules attached to the surface of nanoparticles is determined to range between 0.02 and 6.04 ligands per square nanometer. A significant fraction of the nanoparticles (1.2% injected dose per mass of organ) circulates in the blood after 24 h.Conclusion: EHD co-jetting is a versatile method for the fabrication of nanoparticles for drug delivery. Circulation of the nanoparticles for 24 h is a pre-requisite for subsequent studies to explore defined targeting of the nanoparticles to a specific anatomic site.

Original languageEnglish
Pages (from-to)750-758
Number of pages9
JournalJournal of Drug Targeting
Volume23
Issue number7-8
DOIs
Publication statusPublished - 14 Sept 2015
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2015 Taylor & Francis.

Keywords

  • Biodistribution studies
  • Janus nanoparticles
  • electrospraying
  • functional polymers
  • ligand density

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