Kinetic and microbial response of activated sludge community to acute and chronic exposure to tetracycline

Ilke Pala-Ozkok; Emine Ubay-Cokgor; Daniel Jonas; Derin Orhon

doi:10.1016/j.jhazmat.2018.12.094

Kinetic and microbial response of activated sludge community to acute and chronic exposure to tetracycline

Ilke Pala-Ozkok^*
, Emine Ubay-Cokgor
, Daniel Jonas
, Derin Orhon

^*Corresponding author for this work

Department of Environmental Engineering

Istanbul Technical University
University of Stavanger
University of Freiburg
Near East University

Research output: Contribution to journal › Article › peer-review

35 Citations (Scopus)

Abstract

Current study aimed to discover both kinetic and microbial response of activated sludge biomass to continuous exposure to tetracycline, one of the most frequently detected antibiotics in wastewaters. Respirometric analysis and model evaluation of the oxygen utilization rate profiles generated at critical phases of the experimental period showed that, continuous exposure to tetracycline caused complete suppression of substrate storage aside from mild inhibition on the growth kinetics and it exerted a significant binding action with available organic carbon, leading to less oxygen consumption. Additionally, increase in endogenous decay rates by 1.5 fold was associated with maintenance energy dictated by the presence and production of antibiotic resistance genes, as demonstrated by resistance gene profile. High-throughput sequencing results showed that continuously exposure to tetracycline caused a significant shift in the community structure at species level so that tetracycline resistant bacteria like Arthrobacter sp and Diaphorobacter sp dominated the bacterial community.

Original language	English
Pages (from-to)	418-426
Number of pages	9
Journal	Journal of Hazardous Materials
Volume	367
DOIs	https://doi.org/10.1016/j.jhazmat.2018.12.094
Publication status	Published - 5 Apr 2019

Bibliographical note

Publisher Copyright:
© 2018 Elsevier B.V.

Funding

This study was supported by Turkish Academy of Sciences as part of Fellowship Program for Integrated Doctoral Studies . Authors thank The Scientific Research Fund of Istanbul Technical University (ProjectNr:33680 and ProjectNr:33742) and The Scientific and Technological Research Council of Turkey . Authors thank University of Kiel Institute of Clinical Molecular Biology for their technical assistance.

Funders
Türkiye Bilimsel ve Teknolojik Araştirma Kurumu
Türkiye Bilimler Akademisi
Istanbul Teknik Üniversitesi

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 6 Clean Water and Sanitation

Keywords

Activated sludge modeling
Aerobic processes
Chronic inhibition
High-throughput sequencing
Tetracycline

Access to Document

10.1016/j.jhazmat.2018.12.094

Cite this

@article{370365b404314cd596d8703d37974c72,

title = "Kinetic and microbial response of activated sludge community to acute and chronic exposure to tetracycline",

abstract = "Current study aimed to discover both kinetic and microbial response of activated sludge biomass to continuous exposure to tetracycline, one of the most frequently detected antibiotics in wastewaters. Respirometric analysis and model evaluation of the oxygen utilization rate profiles generated at critical phases of the experimental period showed that, continuous exposure to tetracycline caused complete suppression of substrate storage aside from mild inhibition on the growth kinetics and it exerted a significant binding action with available organic carbon, leading to less oxygen consumption. Additionally, increase in endogenous decay rates by 1.5 fold was associated with maintenance energy dictated by the presence and production of antibiotic resistance genes, as demonstrated by resistance gene profile. High-throughput sequencing results showed that continuously exposure to tetracycline caused a significant shift in the community structure at species level so that tetracycline resistant bacteria like Arthrobacter sp and Diaphorobacter sp dominated the bacterial community.",

keywords = "Activated sludge modeling, Aerobic processes, Chronic inhibition, High-throughput sequencing, Tetracycline",

author = "Ilke Pala-Ozkok and Emine Ubay-Cokgor and Daniel Jonas and Derin Orhon",

note = "Publisher Copyright: {\textcopyright} 2018 Elsevier B.V.",

year = "2019",

month = apr,

day = "5",

doi = "10.1016/j.jhazmat.2018.12.094",

language = "English",

volume = "367",

pages = "418--426",

journal = "Journal of Hazardous Materials",

issn = "0304-3894",

publisher = "Elsevier B.V.",

}

TY - JOUR

T1 - Kinetic and microbial response of activated sludge community to acute and chronic exposure to tetracycline

AU - Pala-Ozkok, Ilke

AU - Ubay-Cokgor, Emine

AU - Jonas, Daniel

AU - Orhon, Derin

PY - 2019/4/5

Y1 - 2019/4/5

N2 - Current study aimed to discover both kinetic and microbial response of activated sludge biomass to continuous exposure to tetracycline, one of the most frequently detected antibiotics in wastewaters. Respirometric analysis and model evaluation of the oxygen utilization rate profiles generated at critical phases of the experimental period showed that, continuous exposure to tetracycline caused complete suppression of substrate storage aside from mild inhibition on the growth kinetics and it exerted a significant binding action with available organic carbon, leading to less oxygen consumption. Additionally, increase in endogenous decay rates by 1.5 fold was associated with maintenance energy dictated by the presence and production of antibiotic resistance genes, as demonstrated by resistance gene profile. High-throughput sequencing results showed that continuously exposure to tetracycline caused a significant shift in the community structure at species level so that tetracycline resistant bacteria like Arthrobacter sp and Diaphorobacter sp dominated the bacterial community.

AB - Current study aimed to discover both kinetic and microbial response of activated sludge biomass to continuous exposure to tetracycline, one of the most frequently detected antibiotics in wastewaters. Respirometric analysis and model evaluation of the oxygen utilization rate profiles generated at critical phases of the experimental period showed that, continuous exposure to tetracycline caused complete suppression of substrate storage aside from mild inhibition on the growth kinetics and it exerted a significant binding action with available organic carbon, leading to less oxygen consumption. Additionally, increase in endogenous decay rates by 1.5 fold was associated with maintenance energy dictated by the presence and production of antibiotic resistance genes, as demonstrated by resistance gene profile. High-throughput sequencing results showed that continuously exposure to tetracycline caused a significant shift in the community structure at species level so that tetracycline resistant bacteria like Arthrobacter sp and Diaphorobacter sp dominated the bacterial community.

KW - Activated sludge modeling

KW - Aerobic processes

KW - Chronic inhibition

KW - High-throughput sequencing

KW - Tetracycline

UR - https://www.scopus.com/pages/publications/85059309803

U2 - 10.1016/j.jhazmat.2018.12.094

DO - 10.1016/j.jhazmat.2018.12.094

M3 - Article

C2 - 30611034

AN - SCOPUS:85059309803

SN - 0304-3894

VL - 367

SP - 418

EP - 426

JO - Journal of Hazardous Materials

JF - Journal of Hazardous Materials

ER -

Kinetic and microbial response of activated sludge community to acute and chronic exposure to tetracycline

Abstract

Bibliographical note

Funding

UN SDGs

Keywords

Access to Document

Other files and links

Fingerprint

Cite this