Isolation and analysis of bioactive diterpenoids in Salvia species (Salvia chionantha and Salvia kronenburgiii) by micellar electrokinetic capillary chromatography

Nevin Öztekin; Selda Başkan; S. Evrim Kepekçi; F. Bedia Erim; Gülaçti Topçu

doi:10.1016/j.jpba.2009.04.012

Isolation and analysis of bioactive diterpenoids in Salvia species (Salvia chionantha and Salvia kronenburgiii) by micellar electrokinetic capillary chromatography

Nevin Öztekin, Selda Başkan, S. Evrim Kepekçi, F. Bedia Erim^*, Gülaçti Topçu

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

29 Citations (Scopus)

Abstract

In the present work, we isolated two bioactive diterpenoids, horminone and 7-O-acetylhorminone and developed a micellar electrokinetic chromatography (MEKC) method for the simultaneous quantitative analysis of them in Turkish Salvia species. The optimal separation electrolyte was 50 mmol/L SDS and 25% methanol at pH 11.5. The limits of detection (S/N = 3) were 3.269 and 4.518 μg/mL for horminone and 7-O-acetylhorminone, respectively. The method has been applied successfully to analyze these two components in Salvia chionantha and Salvia kronenburgii acetone extracts.

Original language	English
Pages (from-to)	439-442
Number of pages	4
Journal	Journal of Pharmaceutical and Biomedical Analysis
Volume	51
Issue number	2
DOIs	https://doi.org/10.1016/j.jpba.2009.04.012
Publication status	Published - 20 Jan 2010

Funding

We thank the TUBITAK (Grant TBA6-2312, 103T053) for financial support.

Funders	Funder number
TUBITAK	103T053, TBA6-2312

Keywords

7-O-acetylhorminone
Capillary electrophoresis
Diterpenoids
Horminone
MEKC
Plant
Salvia

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10.1016/j.jpba.2009.04.012

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title = "Isolation and analysis of bioactive diterpenoids in Salvia species (Salvia chionantha and Salvia kronenburgiii) by micellar electrokinetic capillary chromatography",

abstract = "In the present work, we isolated two bioactive diterpenoids, horminone and 7-O-acetylhorminone and developed a micellar electrokinetic chromatography (MEKC) method for the simultaneous quantitative analysis of them in Turkish Salvia species. The optimal separation electrolyte was 50 mmol/L SDS and 25% methanol at pH 11.5. The limits of detection (S/N = 3) were 3.269 and 4.518 μg/mL for horminone and 7-O-acetylhorminone, respectively. The method has been applied successfully to analyze these two components in Salvia chionantha and Salvia kronenburgii acetone extracts.",

keywords = "7-O-acetylhorminone, Capillary electrophoresis, Diterpenoids, Horminone, MEKC, Plant, Salvia",

author = "Nevin {\"O}ztekin and Selda Ba{\c s}kan and {Evrim Kepek{\c c}i}, S. and Erim, {F. Bedia} and G{\"u}la{\c c}ti Top{\c c}u",

year = "2010",

month = jan,

day = "20",

doi = "10.1016/j.jpba.2009.04.012",

language = "English",

volume = "51",

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journal = "Journal of Pharmaceutical and Biomedical Analysis",

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Isolation and analysis of bioactive diterpenoids in Salvia species (Salvia chionantha and Salvia kronenburgiii) by micellar electrokinetic capillary chromatography. / Öztekin, Nevin; Başkan, Selda; Evrim Kepekçi, S. et al.
In: Journal of Pharmaceutical and Biomedical Analysis, Vol. 51, No. 2, 20.01.2010, p. 439-442.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Isolation and analysis of bioactive diterpenoids in Salvia species (Salvia chionantha and Salvia kronenburgiii) by micellar electrokinetic capillary chromatography

AU - Öztekin, Nevin

AU - Başkan, Selda

AU - Evrim Kepekçi, S.

AU - Erim, F. Bedia

AU - Topçu, Gülaçti

PY - 2010/1/20

Y1 - 2010/1/20

N2 - In the present work, we isolated two bioactive diterpenoids, horminone and 7-O-acetylhorminone and developed a micellar electrokinetic chromatography (MEKC) method for the simultaneous quantitative analysis of them in Turkish Salvia species. The optimal separation electrolyte was 50 mmol/L SDS and 25% methanol at pH 11.5. The limits of detection (S/N = 3) were 3.269 and 4.518 μg/mL for horminone and 7-O-acetylhorminone, respectively. The method has been applied successfully to analyze these two components in Salvia chionantha and Salvia kronenburgii acetone extracts.

AB - In the present work, we isolated two bioactive diterpenoids, horminone and 7-O-acetylhorminone and developed a micellar electrokinetic chromatography (MEKC) method for the simultaneous quantitative analysis of them in Turkish Salvia species. The optimal separation electrolyte was 50 mmol/L SDS and 25% methanol at pH 11.5. The limits of detection (S/N = 3) were 3.269 and 4.518 μg/mL for horminone and 7-O-acetylhorminone, respectively. The method has been applied successfully to analyze these two components in Salvia chionantha and Salvia kronenburgii acetone extracts.

KW - 7-O-acetylhorminone

KW - Capillary electrophoresis

KW - Diterpenoids

KW - Horminone

KW - MEKC

KW - Plant

KW - Salvia

UR - http://www.scopus.com/inward/record.url?scp=70349829857&partnerID=8YFLogxK

U2 - 10.1016/j.jpba.2009.04.012

DO - 10.1016/j.jpba.2009.04.012

M3 - Article

C2 - 19446421

AN - SCOPUS:70349829857

SN - 0731-7085

VL - 51

SP - 439

EP - 442

JO - Journal of Pharmaceutical and Biomedical Analysis

JF - Journal of Pharmaceutical and Biomedical Analysis

IS - 2

ER -

Isolation and analysis of bioactive diterpenoids in Salvia species (Salvia chionantha and Salvia kronenburgiii) by micellar electrokinetic capillary chromatography

Abstract

Funding

Keywords

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