Abstract
The study evaluated impact of sulfamethoxazole on acetate utilization kinetics and microbial community structure using respirometric analysis and pyrosequencing. A fill and draw reactor fed with acetate was sustained at a sludge age of 10 days. Acute impact was assessed by modeling of respirometric data in batch reactors started with sulfamethoxazole doses in the range of 25-200 mg/L. Fill and draw operation resumed with continuous sulfamethoxazole dosing of 50 mg/L and the chronic impact was evaluated with acclimated biomass after 20 days. Acute impact revealed higher maintenance energy requirements, activity reduction and slight substrate binding. Chronic impact resulted in retardation of substrate storage. A fraction of acetate was utilized at a much lower rate with partial biodegradation of sulfamethoxazole by the acclimated biomass. Pyrosequencing indicated that Amaricoccus sp. and an unclassified Bacteroidetes sp., possibly with the ability to co-metabolize sulfamethoxazole, dominated the community.
| Original language | English |
|---|---|
| Pages (from-to) | 65-76 |
| Number of pages | 12 |
| Journal | Bioresource Technology |
| Volume | 206 |
| DOIs | |
| Publication status | Published - 1 Apr 2016 |
Bibliographical note
Publisher Copyright:© 2016 Elsevier Ltd.
Funding
This study was supported by the Turkish Academy of Sciences as part of Fellowship Program for Integrated Doctoral Studies. Authors thank The Scientific Research Fund of Istanbul Technical University (Project Nr: 34235 and 34117) and The Scientific and Technological Research Council of Turkey. Authors thank University of Kiel Institute of Clinical Molecular Biology for the technical assistance.
| Funders | Funder number |
|---|---|
| Türkiye Bilimsel ve Teknolojik Araştirma Kurumu | |
| Türkiye Bilimler Akademisi | |
| Istanbul Teknik Üniversitesi | 34117, 34235 |
Keywords
- 454-Pyrosequencing
- Biodegradation
- Inhibitory impact
- Process modeling
- Sulfamethoxazole