Investigation on the effects of variable shear stress on monocyte cell morphology

Semra Z. Birol, Rana Fucucuoglu, Sertac Cadirci, Ayca S. Yazgan, Levent Trabzon

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

2 Citations (Scopus)

Abstract

Changes in flow shear stress are sensed by cell membrane receptors transferring to intracellular area that lead to physical and chemical alterations. This process is defined as mechonatransduction process that is essential for maintaining the development of cells and tissues. Vascular endothelial cells and blood cells in circulation subjected to shear stress on different levels in different regions of vascular network. There have been platforms those are fabricated by different techniques to perform in vitro cell culture experiments to reveal shear stress effects on cells. Microchannels based on PDMS fabricating by soft lithography technique have been favourable in mechanobiological applications as using for cell culture platforms, especially shear stress studies. Normally, physiological shear stress levels for veins are between 10 to 70 dynes/cm2 (1-7 Pa) and for arteries between 1 to 6 dynes/cm2 (0.1-0.7 Pa). In this study, a circular system has been set up by microfluidic pump to mimic physiological environment of monocyte cells for monitoring structural changes at high level shear stress. Shear stress at 12 Pa more than physiological level simulated by CFD has been applied on 8×106/10 mL HL-60 cells under 3600 μL/min. during 3 hours. Cells were passed through cavitated PDMS channel. Then, cells were stained with Phalloidin-TRITC and counterstained with DAPI to monitoring fluorescence of F-Actin molecules.

Original languageEnglish
Title of host publication2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages239-242
Number of pages4
ISBN (Electronic)9781509030590
DOIs
Publication statusPublished - 25 Aug 2017
Event12th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017 - Los Angeles, United States
Duration: 9 Apr 201712 Apr 2017

Publication series

Name2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017

Conference

Conference12th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017
Country/TerritoryUnited States
CityLos Angeles
Period9/04/1712/04/17

Bibliographical note

Publisher Copyright:
© 2017 IEEE.

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