TY - GEN
T1 - Investigation of nanophysical properties of aging polyamide nanofibrillar tissue scaffolds by TEM, SAED, contact angle and Raman spectroscopies
AU - Ayres, Virginia M.
AU - Xie, Kan
AU - Tiryaki, Volkan Mujdat
AU - Ahmed, Ijaz
AU - Shreiber, David I.
PY - 2012
Y1 - 2012
N2 - The nanoscale physical properties of newly electrospun polyamide nanofibrillar matrices < 1 year old versus those that were > 3 year old were investigated with transmission electron microscopy, selected area electron diffraction, contact angle measurements, and Raman spectroscopy. Significant differences in crystallinity, hydrophobicity, and chemistry were found and correspondingly different cell responses by cerebellar granular neurons were observed. The properties of the aged nanofibrillar scaffolds evoked a response for neuron burrowing into a more 3-dimensional environment in addition to better facilitation of neurite outgrowth. The nanophysical properties of tissue scaffolds have been recently shown to directly and indirectly regulate cellular responses. As physical properties can evolve over time, the present investigation addresses the issue of tissue scaffold shelf life, with possible changes in directive signals to cells.
AB - The nanoscale physical properties of newly electrospun polyamide nanofibrillar matrices < 1 year old versus those that were > 3 year old were investigated with transmission electron microscopy, selected area electron diffraction, contact angle measurements, and Raman spectroscopy. Significant differences in crystallinity, hydrophobicity, and chemistry were found and correspondingly different cell responses by cerebellar granular neurons were observed. The properties of the aged nanofibrillar scaffolds evoked a response for neuron burrowing into a more 3-dimensional environment in addition to better facilitation of neurite outgrowth. The nanophysical properties of tissue scaffolds have been recently shown to directly and indirectly regulate cellular responses. As physical properties can evolve over time, the present investigation addresses the issue of tissue scaffold shelf life, with possible changes in directive signals to cells.
UR - http://www.scopus.com/inward/record.url?scp=84879380465&partnerID=8YFLogxK
U2 - 10.1557/opl.2012.747
DO - 10.1557/opl.2012.747
M3 - Conference contribution
AN - SCOPUS:84879380465
SN - 9781627482295
T3 - Materials Research Society Symposium Proceedings
SP - 68
EP - 73
BT - Biomaterials for Tissue Regeneration
T2 - 2011 MRS Fall Meeting
Y2 - 28 November 2011 through 2 December 2011
ER -