Investigation of biodegradability and cellular activity of PCL/PLA and PCL/PLLA electrospun webs for tissue engineering applications

Janset Oztemur*, Suzan Ozdemir, Havva Tezcan-Unlu, Gulsah Cecener, Hande Sezgin, Ipek Yalcin-Enis

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Biodegradability and cellular activity are key performance indicators that should be prioritized for tissue engineering applications. Biopolymer selection, determination of necessary structural properties, and their synergistic interactions play an active role in obtaining the expected biodegradability and biological activity from scaffolds. In this study, it is aimed to produce electrospun webs with improved biocompatibility by blending polycaprolactone (PCL) with polylactic acid (PLA) and poly-l-lactide (PLLA), and examine the effect of biopolymer selection and blend ratio on the biodegradability and cellular activity of surfaces. In this context, fibrous webs are produced from PCL/PLA and PCL/PLLA blends with a weight ratio of 80/20 and 50/50 and pure polymers of PCL, PLA, and PLLA by electrospinning method and subjected to morphological and biological analyses. The biodegradation tests are carried out hydrolytically while the cell viability and cell proliferation analyses are performed with adult human primary dermal fibroblasts and human umbilical endothelial cells (HUVECs). The results show that the fiber diameters of the fabricated webs ranged from 0.747 to 1.685 μm. At the end of the 5th month, it is observed that the biodegradation rates of the webs blended 50% with PLA and PLLA, in comparison to PCL ones, increase from 3.7% to 13.33% and 7.69%, respectively. On the other hand, cell culture results highlight that the addition of 20% PLA and PLLA improves the cellular activity of both cell types, but increased PLA or PLLA ratio in PCL webs has a negative effect as it makes the structure stiff and brittle.

Original languageEnglish
Article numbere23564
JournalBiopolymers
Volume114
Issue number11
DOIs
Publication statusPublished - Nov 2023

Bibliographical note

Publisher Copyright:
© 2023 Wiley Periodicals LLC.

Funding

This study is supported by the TUBITAK (The Scientific and Technological Research Council of Turkey) under grant no: 121M309 and ITU Scientific Research Projects under grant no: 43368. HTU is a 100/2000 The Council of Higher Education (CoHE) PhD scholar in Molecular Oncology. This study is supported by the TUBITAK (The Scientific and Technological Research Council of Turkey) under grant no: 121M309 and ITU Scientific Research Projects under grant no: 43368. HTU is a 100/2000 The Council of Higher Education (CoHE) PhD scholar in Molecular Oncology.

FundersFunder number
International Technological University43368
Türkiye Bilimsel ve Teknolojik Araştırma Kurumu121M309
Yükseköğretim Kurulu

    Keywords

    • biodegradation
    • biopolymers
    • cell proliferation
    • electrospinning

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