Integrated Microfluidic Extraction and Detection of Aflatoxin B1 in Olive Oil

Food microfluidics are powerful tools to create total analysis systems and have long been demonstrated to be useful for safety and quality applications. This study introduced a new integrated aflatoxin B1 extraction and detection system based on microfluidic technology. A poly(dimethylsiloxane) microfluidic mixer was developed herein to rapidly extract aflatoxin B1 from olive oil samples. We believe that the sunflower microfluidic mixer developed in this study can be widely applied in a variety of related fields that are extremely difficult in traditional batch processes. Successful integration of microfluidic mixers brings us closer to one-step detection. The used approach allows for analysis with only 2 ml of sample. The disposable and cost-effective paper-based microfluidics were utilized to construct the immunosensor for aflatoxin B1 detection. Owing to the high surface area of paper and carbon nanotubes sensitive measurement can be done at le 0.01 nanogram levels, below the regulatory requirements. Further, the paper-based microfluidic exhibited high recoveries between 91 and 97% for aflatoxin B1 detection in olive oil, demonstrating the method’s potential for use in the study of a variety of agricultural and culinary goods.