Improved Candida methylica formate dehydrogenase fermentation through statistical optimization of low-cost culture media

Mustafa Kahraman, Emel Biçakçi Ordu, Koray Yeşiladali, Nevin Gül Karagüler*, Candan Tamerler

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

NAD+-dependent formate dehydrogenase (FDH, EC 1.2.1.2) is of use in the regeneration of NAD(P)H coenzymes, and therefore has strong potential for practical application in chemical and medical industries. A low-cost production of recombinant Escherichia coli (E. coli) containing FDH from Candida methylica (cmFDH) was optimized in molasses-based medium by using response surface methodology (RSM) based on central composite design (CCD). The beet molasses as a sole carbon source, (NH4)2HPO4 as a nitrogen and phosphorus source, KH2PO4 as a buffer agent, and Mg2SO4 · 7H2O as a magnesium and sulfur source were used as variables in the medium. The optimum medium composition was found to be 34.694gL-1 of reducing sugar (equivalent to molasses solution), 8.536gL-1 of (NH4) 2HPO4, 3.073gL-1 of KH2PO 4, and 1.707gL-1 of Mg2SO4 · 7H2O. Molasses-based culture medium increased the yield of cmFDH about three times compared to LB medium. The currently developed media has the potential to be used in industrial bioprocesses with low-cost production.

Original languageEnglish
Pages (from-to)507-519
Number of pages13
JournalPreparative Biochemistry and Biotechnology
Volume42
Issue number6
DOIs
Publication statusPublished - 1 Nov 2012

Keywords

  • biocatalyst preparation
  • Candida methylica
  • culture media
  • formate dehydrogenase
  • statistical optimization

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