Improved Candida methylica formate dehydrogenase fermentation through statistical optimization of low-cost culture media

Mustafa Kahraman, Emel Biçakçi Ordu, Koray Yeşiladali, Nevin Gül Karagüler*, Candan Tamerler

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

NAD+-dependent formate dehydrogenase (FDH, EC 1.2.1.2) is of use in the regeneration of NAD(P)H coenzymes, and therefore has strong potential for practical application in chemical and medical industries. A low-cost production of recombinant Escherichia coli (E. coli) containing FDH from Candida methylica (cmFDH) was optimized in molasses-based medium by using response surface methodology (RSM) based on central composite design (CCD). The beet molasses as a sole carbon source, (NH4)2HPO4 as a nitrogen and phosphorus source, KH2PO4 as a buffer agent, and Mg2SO4 · 7H2O as a magnesium and sulfur source were used as variables in the medium. The optimum medium composition was found to be 34.694gL-1 of reducing sugar (equivalent to molasses solution), 8.536gL-1 of (NH4) 2HPO4, 3.073gL-1 of KH2PO 4, and 1.707gL-1 of Mg2SO4 · 7H2O. Molasses-based culture medium increased the yield of cmFDH about three times compared to LB medium. The currently developed media has the potential to be used in industrial bioprocesses with low-cost production.

Original languageEnglish
Pages (from-to)507-519
Number of pages13
JournalPreparative Biochemistry and Biotechnology
Volume42
Issue number6
DOIs
Publication statusPublished - 1 Nov 2012

Funding

This work was partially supported by the Turkish State Planning Organization’s Advanced Technologies Program and by the Turkish State Planning Organization (project 90188).

FundersFunder number
Turkish State Planning Organization90188

    Keywords

    • biocatalyst preparation
    • Candida methylica
    • culture media
    • formate dehydrogenase
    • statistical optimization

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