TY - JOUR
T1 - Improved Candida methylica formate dehydrogenase fermentation through statistical optimization of low-cost culture media
AU - Kahraman, Mustafa
AU - Ordu, Emel Biçakçi
AU - Yeşiladali, Koray
AU - Karagüler, Nevin Gül
AU - Tamerler, Candan
PY - 2012/11/1
Y1 - 2012/11/1
N2 - NAD+-dependent formate dehydrogenase (FDH, EC 1.2.1.2) is of use in the regeneration of NAD(P)H coenzymes, and therefore has strong potential for practical application in chemical and medical industries. A low-cost production of recombinant Escherichia coli (E. coli) containing FDH from Candida methylica (cmFDH) was optimized in molasses-based medium by using response surface methodology (RSM) based on central composite design (CCD). The beet molasses as a sole carbon source, (NH4)2HPO4 as a nitrogen and phosphorus source, KH2PO4 as a buffer agent, and Mg2SO4 · 7H2O as a magnesium and sulfur source were used as variables in the medium. The optimum medium composition was found to be 34.694gL-1 of reducing sugar (equivalent to molasses solution), 8.536gL-1 of (NH4) 2HPO4, 3.073gL-1 of KH2PO 4, and 1.707gL-1 of Mg2SO4 · 7H2O. Molasses-based culture medium increased the yield of cmFDH about three times compared to LB medium. The currently developed media has the potential to be used in industrial bioprocesses with low-cost production.
AB - NAD+-dependent formate dehydrogenase (FDH, EC 1.2.1.2) is of use in the regeneration of NAD(P)H coenzymes, and therefore has strong potential for practical application in chemical and medical industries. A low-cost production of recombinant Escherichia coli (E. coli) containing FDH from Candida methylica (cmFDH) was optimized in molasses-based medium by using response surface methodology (RSM) based on central composite design (CCD). The beet molasses as a sole carbon source, (NH4)2HPO4 as a nitrogen and phosphorus source, KH2PO4 as a buffer agent, and Mg2SO4 · 7H2O as a magnesium and sulfur source were used as variables in the medium. The optimum medium composition was found to be 34.694gL-1 of reducing sugar (equivalent to molasses solution), 8.536gL-1 of (NH4) 2HPO4, 3.073gL-1 of KH2PO 4, and 1.707gL-1 of Mg2SO4 · 7H2O. Molasses-based culture medium increased the yield of cmFDH about three times compared to LB medium. The currently developed media has the potential to be used in industrial bioprocesses with low-cost production.
KW - biocatalyst preparation
KW - Candida methylica
KW - culture media
KW - formate dehydrogenase
KW - statistical optimization
UR - http://www.scopus.com/inward/record.url?scp=84867344847&partnerID=8YFLogxK
U2 - 10.1080/10826068.2012.659102
DO - 10.1080/10826068.2012.659102
M3 - Article
C2 - 23030463
AN - SCOPUS:84867344847
SN - 1082-6068
VL - 42
SP - 507
EP - 519
JO - Preparative Biochemistry and Biotechnology
JF - Preparative Biochemistry and Biotechnology
IS - 6
ER -