Immobilization of polyphenol oxidase in conducting graft copolymers and determination of phenolic amount in red wines with enzyme electrodes

Huseyin Bekir Yildiz; Levent Toppare; Yesim Hepuzer Gursel; Yusuf Yagci

doi:10.1016/j.enzmictec.2006.01.026

Immobilization of polyphenol oxidase in conducting graft copolymers and determination of phenolic amount in red wines with enzyme electrodes

Huseyin Bekir Yildiz, Levent Toppare^*, Yesim Hepuzer Gursel, Yusuf Yagci

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

Polyphenol oxidase was immobilized in several conducting copolymer matrices. Three different types of poly(methyl methacrylate-co-methyl thienyl methacrylate) matrices were used to obtain copolymers. Immobilization of enzyme was carried out by the entrapment of the enzyme in conducting copolymer matrices during electrochemical polymerization of pyrrole through thiophene moieties of the polymers. Maximum reaction rates, Michaelis-Menten constants, optimum temperature and pH values and operational stabilities of the enzyme electrodes were investigated. Total amount of phenolic compounds in some Turkish red wines was analyzed using these electrodes.

Original language	English
Pages (from-to)	945-948
Number of pages	4
Journal	Enzyme and Microbial Technology
Volume	39
Issue number	4
DOIs	https://doi.org/10.1016/j.enzmictec.2006.01.026
Publication status	Published - 2 Aug 2006

Funding

This was partially supported by DPT-2005K120580 and TUBA grants. Authors greatfully thank Dr. Senem Kiralp for valuable discussions.

Funders	Funder number
TUBA

Keywords

Electrochemistry
Immobilization
Polyphenol oxidase
Polyphenols
Red wines

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10.1016/j.enzmictec.2006.01.026

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title = "Immobilization of polyphenol oxidase in conducting graft copolymers and determination of phenolic amount in red wines with enzyme electrodes",

abstract = "Polyphenol oxidase was immobilized in several conducting copolymer matrices. Three different types of poly(methyl methacrylate-co-methyl thienyl methacrylate) matrices were used to obtain copolymers. Immobilization of enzyme was carried out by the entrapment of the enzyme in conducting copolymer matrices during electrochemical polymerization of pyrrole through thiophene moieties of the polymers. Maximum reaction rates, Michaelis-Menten constants, optimum temperature and pH values and operational stabilities of the enzyme electrodes were investigated. Total amount of phenolic compounds in some Turkish red wines was analyzed using these electrodes.",

keywords = "Electrochemistry, Immobilization, Polyphenol oxidase, Polyphenols, Red wines",

author = "Yildiz, {Huseyin Bekir} and Levent Toppare and Gursel, {Yesim Hepuzer} and Yusuf Yagci",

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T1 - Immobilization of polyphenol oxidase in conducting graft copolymers and determination of phenolic amount in red wines with enzyme electrodes

AU - Yildiz, Huseyin Bekir

AU - Toppare, Levent

AU - Gursel, Yesim Hepuzer

AU - Yagci, Yusuf

PY - 2006/8/2

Y1 - 2006/8/2

N2 - Polyphenol oxidase was immobilized in several conducting copolymer matrices. Three different types of poly(methyl methacrylate-co-methyl thienyl methacrylate) matrices were used to obtain copolymers. Immobilization of enzyme was carried out by the entrapment of the enzyme in conducting copolymer matrices during electrochemical polymerization of pyrrole through thiophene moieties of the polymers. Maximum reaction rates, Michaelis-Menten constants, optimum temperature and pH values and operational stabilities of the enzyme electrodes were investigated. Total amount of phenolic compounds in some Turkish red wines was analyzed using these electrodes.

AB - Polyphenol oxidase was immobilized in several conducting copolymer matrices. Three different types of poly(methyl methacrylate-co-methyl thienyl methacrylate) matrices were used to obtain copolymers. Immobilization of enzyme was carried out by the entrapment of the enzyme in conducting copolymer matrices during electrochemical polymerization of pyrrole through thiophene moieties of the polymers. Maximum reaction rates, Michaelis-Menten constants, optimum temperature and pH values and operational stabilities of the enzyme electrodes were investigated. Total amount of phenolic compounds in some Turkish red wines was analyzed using these electrodes.

KW - Electrochemistry

KW - Immobilization

KW - Polyphenol oxidase

KW - Polyphenols

KW - Red wines

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U2 - 10.1016/j.enzmictec.2006.01.026

DO - 10.1016/j.enzmictec.2006.01.026

M3 - Article

AN - SCOPUS:33745223514

SN - 0141-0229

VL - 39

SP - 945

EP - 948

JO - Enzyme and Microbial Technology

JF - Enzyme and Microbial Technology

IS - 4

ER -

Immobilization of polyphenol oxidase in conducting graft copolymers and determination of phenolic amount in red wines with enzyme electrodes

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