Immobilization of invertase in conducting thiophene-capped poly(methylmethacrylate)/polypyrrole matrices

Selmiye Alkan; Levent Toppare; Yusuf Yagci; Yesim Hepuzer

doi:10.1163/156856299X00036

Immobilization of invertase in conducting thiophene-capped poly(methylmethacrylate)/polypyrrole matrices

Selmiye Alkan, Levent Toppare^*, Yusuf Yagci, Yesim Hepuzer

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

31 Citations (Scopus)

Abstract

Immobilization of invertase in thiophene-capped poly(methylmethacrylate)/polypyrrole matrices was achieved by constant potential electrolysis using different supporting electrolytes. Optimum reaction conditions such as substrate concentration, temperature, and pH for the enzyme electrodes were determined. The temperature and pH were found to be 60°C and 4.8, respectively. The effect of supporting electrolyte on the enzyme activity revealed that SDS was the best in the immobilization procedure. Michaelis-Menten constant and the maximum reaction rate in PMMA/PPy matrices were of the order of that of pristine polypyrrole. However, in terms of repeated use, the copolymer matrices were superior to polypyrrole.

Original language	English
Pages (from-to)	1223-1235
Number of pages	13
Journal	Journal of Biomaterials Science, Polymer Edition
Volume	10
Issue number	12
DOIs	https://doi.org/10.1163/156856299X00036
Publication status	Published - 1 Jan 1999

Keywords

Electropolymerization
Immobilization
Invertase
Poly(methylmethacrylate)
Polypyrrole

Access to Document

10.1163/156856299X00036

Cite this

@article{49c2cb37bc8f49c390deeb11a0e3f9b5,

title = "Immobilization of invertase in conducting thiophene-capped poly(methylmethacrylate)/polypyrrole matrices",

abstract = "Immobilization of invertase in thiophene-capped poly(methylmethacrylate)/polypyrrole matrices was achieved by constant potential electrolysis using different supporting electrolytes. Optimum reaction conditions such as substrate concentration, temperature, and pH for the enzyme electrodes were determined. The temperature and pH were found to be 60°C and 4.8, respectively. The effect of supporting electrolyte on the enzyme activity revealed that SDS was the best in the immobilization procedure. Michaelis-Menten constant and the maximum reaction rate in PMMA/PPy matrices were of the order of that of pristine polypyrrole. However, in terms of repeated use, the copolymer matrices were superior to polypyrrole.",

keywords = "Electropolymerization, Immobilization, Invertase, Poly(methylmethacrylate), Polypyrrole",

author = "Selmiye Alkan and Levent Toppare and Yusuf Yagci and Yesim Hepuzer",

year = "1999",

month = jan,

day = "1",

doi = "10.1163/156856299X00036",

language = "English",

volume = "10",

pages = "1223--1235",

journal = "Journal of Biomaterials Science, Polymer Edition",

issn = "0920-5063",

publisher = "Taylor and Francis Ltd.",

number = "12",

}

TY - JOUR

T1 - Immobilization of invertase in conducting thiophene-capped poly(methylmethacrylate)/polypyrrole matrices

AU - Alkan, Selmiye

AU - Toppare, Levent

AU - Yagci, Yusuf

AU - Hepuzer, Yesim

PY - 1999/1/1

Y1 - 1999/1/1

N2 - Immobilization of invertase in thiophene-capped poly(methylmethacrylate)/polypyrrole matrices was achieved by constant potential electrolysis using different supporting electrolytes. Optimum reaction conditions such as substrate concentration, temperature, and pH for the enzyme electrodes were determined. The temperature and pH were found to be 60°C and 4.8, respectively. The effect of supporting electrolyte on the enzyme activity revealed that SDS was the best in the immobilization procedure. Michaelis-Menten constant and the maximum reaction rate in PMMA/PPy matrices were of the order of that of pristine polypyrrole. However, in terms of repeated use, the copolymer matrices were superior to polypyrrole.

AB - Immobilization of invertase in thiophene-capped poly(methylmethacrylate)/polypyrrole matrices was achieved by constant potential electrolysis using different supporting electrolytes. Optimum reaction conditions such as substrate concentration, temperature, and pH for the enzyme electrodes were determined. The temperature and pH were found to be 60°C and 4.8, respectively. The effect of supporting electrolyte on the enzyme activity revealed that SDS was the best in the immobilization procedure. Michaelis-Menten constant and the maximum reaction rate in PMMA/PPy matrices were of the order of that of pristine polypyrrole. However, in terms of repeated use, the copolymer matrices were superior to polypyrrole.

KW - Electropolymerization

KW - Immobilization

KW - Invertase

KW - Poly(methylmethacrylate)

KW - Polypyrrole

UR - http://www.scopus.com/inward/record.url?scp=0033399124&partnerID=8YFLogxK

U2 - 10.1163/156856299X00036

DO - 10.1163/156856299X00036

M3 - Article

C2 - 10673018

AN - SCOPUS:0033399124

SN - 0920-5063

VL - 10

SP - 1223

EP - 1235

JO - Journal of Biomaterials Science, Polymer Edition

JF - Journal of Biomaterials Science, Polymer Edition

IS - 12

ER -

Immobilization of invertase in conducting thiophene-capped poly(methylmethacrylate)/polypyrrole matrices

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this