Immobilization of invertase in conducting copolymers of 3-methylthienyl methacrylate

A. Cirpan, S. Alkan, L. Toppare*, Y. Hepuzer, Y. Yagci

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

45 Citations (Scopus)

Abstract

Immobilization of invertase in conducting copolymer matrices of 3-methylthienyl methacrylate with pyrrole and thiophene was achieved by constant potential electrolysis using sodium dodecyl sulfate (SDS) as the supporting electrolyte. Polythiophene (PTh) was also used in entrapment process for comparison. Kinetic parameters, Michaelis-Menten constant, Km, and the maximum reaction rate, Vmax, were investigated. Operational stability and temperature optimization of the enzyme electrodes were also examined.

Original languageEnglish
Pages (from-to)29-33
Number of pages5
JournalBioelectrochemistry
Volume59
Issue number1-2
DOIs
Publication statusPublished - Apr 2003

Keywords

  • Conducting copolymer
  • Electropolymerization
  • Immobilization
  • Invertase
  • Polypyrrole
  • Polythiophene

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