Immobilization of glucose oxidase in polypyrrole/polytetrahydrofuran graft copolymers

S. Tirkeş, L. Toppare*, S. Alkan, U. Bakir, A. Önen, Y. Yǎci

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

34 Citations (Scopus)


Glucose oxidase (GOD) was immobilized in four different conducting polymer matrices, namely: polypyrrole, (PPy), poly(pyrrole-graft-polytetrahydrofuran), (1) and (3); and poly(pyrrole-graft-polystyrene/polytetrahydrofuran), (2). The kinetic parameters Vmax and Km, and the optimum temperature were determined for both immobilized and native enzymes. The effect of electrolysis time and several supporting electrolytes, p-toluenesulfonic acid, p-toluene sulfonic acid (PTSA), sodium p-toluene sulfonate, sodium p-toluene sulfonate (NaPTS), and sodium dodecyl sulfate, sodium dodecyl sulfate (SDS), on enzyme immobilization were investigated. The high Km value (59.9 mM) of enzyme immobilized in PPy was decreased via immobilization in graft copolymer matrices of pyrrole. Vmax, which was 2.25 mM/min for pure PPy, was found as 4.71 mM/min for compound (3).

Original languageEnglish
Pages (from-to)81-87
Number of pages7
JournalInternational Journal of Biological Macromolecules
Issue number2
Publication statusPublished - 8 Apr 2002


This study was supported by State Planning Organization of Turkey (contract no: 98 K 122750) and TUBA.

FundersFunder number
State Planning Organization of Turkey98 K 122750


    • Conductive polymers
    • Glucose oxidase
    • Immobilization


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