Immobilization of glucose oxidase in polypyrrole/polytetrahydrofuran graft copolymers

S. Tirkeş; L. Toppare; S. Alkan; U. Bakir; A. Önen; Y. Yǎci

doi:10.1016/S0141-8130(02)00011-9

Immobilization of glucose oxidase in polypyrrole/polytetrahydrofuran graft copolymers

S. Tirkeş, L. Toppare^*, S. Alkan, U. Bakir, A. Önen, Y. Yǎci

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

34 Citations (Scopus)

Abstract

Glucose oxidase (GOD) was immobilized in four different conducting polymer matrices, namely: polypyrrole, (PPy), poly(pyrrole-graft-polytetrahydrofuran), (1) and (3); and poly(pyrrole-graft-polystyrene/polytetrahydrofuran), (2). The kinetic parameters V_max and K_m, and the optimum temperature were determined for both immobilized and native enzymes. The effect of electrolysis time and several supporting electrolytes, p-toluenesulfonic acid, p-toluene sulfonic acid (PTSA), sodium p-toluene sulfonate, sodium p-toluene sulfonate (NaPTS), and sodium dodecyl sulfate, sodium dodecyl sulfate (SDS), on enzyme immobilization were investigated. The high K_m value (59.9 mM) of enzyme immobilized in PPy was decreased via immobilization in graft copolymer matrices of pyrrole. V_max, which was 2.25 mM/min for pure PPy, was found as 4.71 mM/min for compound (3).

Original language	English
Pages (from-to)	81-87
Number of pages	7
Journal	International Journal of Biological Macromolecules
Volume	30
Issue number	2
DOIs	https://doi.org/10.1016/S0141-8130(02)00011-9
Publication status	Published - 8 Apr 2002

Funding

This study was supported by State Planning Organization of Turkey (contract no: 98 K 122750) and TUBA.

Funders	Funder number
State Planning Organization of Turkey	98 K 122750
TUBA

Keywords

Conductive polymers
Glucose oxidase
Immobilization

Access to Document

10.1016/S0141-8130(02)00011-9

Cite this

@article{e4fe41d800ed43c9a115eeba2ab83613,

title = "Immobilization of glucose oxidase in polypyrrole/polytetrahydrofuran graft copolymers",

abstract = "Glucose oxidase (GOD) was immobilized in four different conducting polymer matrices, namely: polypyrrole, (PPy), poly(pyrrole-graft-polytetrahydrofuran), (1) and (3); and poly(pyrrole-graft-polystyrene/polytetrahydrofuran), (2). The kinetic parameters Vmax and Km, and the optimum temperature were determined for both immobilized and native enzymes. The effect of electrolysis time and several supporting electrolytes, p-toluenesulfonic acid, p-toluene sulfonic acid (PTSA), sodium p-toluene sulfonate, sodium p-toluene sulfonate (NaPTS), and sodium dodecyl sulfate, sodium dodecyl sulfate (SDS), on enzyme immobilization were investigated. The high Km value (59.9 mM) of enzyme immobilized in PPy was decreased via immobilization in graft copolymer matrices of pyrrole. Vmax, which was 2.25 mM/min for pure PPy, was found as 4.71 mM/min for compound (3).",

keywords = "Conductive polymers, Glucose oxidase, Immobilization",

author = "S. Tirke{\c s} and L. Toppare and S. Alkan and U. Bakir and A. {\"O}nen and Y. Yǎci",

year = "2002",

month = apr,

day = "8",

doi = "10.1016/S0141-8130(02)00011-9",

language = "English",

volume = "30",

pages = "81--87",

journal = "International Journal of Biological Macromolecules",

issn = "0141-8130",

publisher = "Elsevier B.V.",

number = "2",

}

TY - JOUR

T1 - Immobilization of glucose oxidase in polypyrrole/polytetrahydrofuran graft copolymers

AU - Tirkeş, S.

AU - Toppare, L.

AU - Alkan, S.

AU - Bakir, U.

AU - Önen, A.

AU - Yǎci, Y.

PY - 2002/4/8

Y1 - 2002/4/8

N2 - Glucose oxidase (GOD) was immobilized in four different conducting polymer matrices, namely: polypyrrole, (PPy), poly(pyrrole-graft-polytetrahydrofuran), (1) and (3); and poly(pyrrole-graft-polystyrene/polytetrahydrofuran), (2). The kinetic parameters Vmax and Km, and the optimum temperature were determined for both immobilized and native enzymes. The effect of electrolysis time and several supporting electrolytes, p-toluenesulfonic acid, p-toluene sulfonic acid (PTSA), sodium p-toluene sulfonate, sodium p-toluene sulfonate (NaPTS), and sodium dodecyl sulfate, sodium dodecyl sulfate (SDS), on enzyme immobilization were investigated. The high Km value (59.9 mM) of enzyme immobilized in PPy was decreased via immobilization in graft copolymer matrices of pyrrole. Vmax, which was 2.25 mM/min for pure PPy, was found as 4.71 mM/min for compound (3).

AB - Glucose oxidase (GOD) was immobilized in four different conducting polymer matrices, namely: polypyrrole, (PPy), poly(pyrrole-graft-polytetrahydrofuran), (1) and (3); and poly(pyrrole-graft-polystyrene/polytetrahydrofuran), (2). The kinetic parameters Vmax and Km, and the optimum temperature were determined for both immobilized and native enzymes. The effect of electrolysis time and several supporting electrolytes, p-toluenesulfonic acid, p-toluene sulfonic acid (PTSA), sodium p-toluene sulfonate, sodium p-toluene sulfonate (NaPTS), and sodium dodecyl sulfate, sodium dodecyl sulfate (SDS), on enzyme immobilization were investigated. The high Km value (59.9 mM) of enzyme immobilized in PPy was decreased via immobilization in graft copolymer matrices of pyrrole. Vmax, which was 2.25 mM/min for pure PPy, was found as 4.71 mM/min for compound (3).

KW - Conductive polymers

KW - Glucose oxidase

KW - Immobilization

UR - http://www.scopus.com/inward/record.url?scp=0037041209&partnerID=8YFLogxK

U2 - 10.1016/S0141-8130(02)00011-9

DO - 10.1016/S0141-8130(02)00011-9

M3 - Article

C2 - 11911897

AN - SCOPUS:0037041209

SN - 0141-8130

VL - 30

SP - 81

EP - 87

JO - International Journal of Biological Macromolecules

JF - International Journal of Biological Macromolecules

IS - 2

ER -

Immobilization of glucose oxidase in polypyrrole/polytetrahydrofuran graft copolymers

Abstract

Funding

Keywords

Access to Document

Other files and links

Fingerprint

Cite this