Immobilization and stabilization of papain on poly(hydroxyethyl methacrylate-ethylenglycol dimethacrylate) beads grafted with epoxy functional polymer chains via surface-initiated-atom transfer radical polymerization (SI-ATRP)

Gulay Bayramoglu*, B. Filiz Senkal, Meltem Yilmaz, M. Yakup Arica

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

38 Citations (Scopus)

Abstract

Poly(hydroxyethyl methacrylate-ethylen glycol dimethacrylate), p(HEMA-EGDMA), beads were prepared by suspension polymerization, and were decorated with fibrous poly(glycidyl methacrylate), p(GMA), via surface initiated-atom transfer radical polymerization (SI-ATRP). The functional epoxy groups of the beads were used for covalent immobilization of papain. The average amount of immobilized enzyme was 18.7mg/g beads. The immobilized enzyme was characterized by temperature, pH, operational and storage stability experiments. The maximum velocity of the free and immobilized enzymes (V max) and Michaelis-Menten constant (K m) values were determined as 10.7 and 8.3U/mg proteins and 274 and 465μM, respectively. The immobilized papain was operated in a batch reactor, and it was very effective for hydrolysis of different proteins (i.e., casein and cytochrom c).

Original languageEnglish
Pages (from-to)9833-9837
Number of pages5
JournalBioresource Technology
Volume102
Issue number21
DOIs
Publication statusPublished - Nov 2011

Keywords

  • Beads
  • Immobilized enzyme
  • Papain
  • Protein hydrolysis
  • SI-ATRP

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