Immobilization and stabilization of papain on poly(hydroxyethyl methacrylate-ethylenglycol dimethacrylate) beads grafted with epoxy functional polymer chains via surface-initiated-atom transfer radical polymerization (SI-ATRP)

Gulay Bayramoglu; B. Filiz Senkal; Meltem Yilmaz; M. Yakup Arica

doi:10.1016/j.biortech.2011.08.042

Immobilization and stabilization of papain on poly(hydroxyethyl methacrylate-ethylenglycol dimethacrylate) beads grafted with epoxy functional polymer chains via surface-initiated-atom transfer radical polymerization (SI-ATRP)

Gulay Bayramoglu^*, B. Filiz Senkal, Meltem Yilmaz, M. Yakup Arica

^*Corresponding author for this work

Department of Chemistry

Gazi University

Research output: Contribution to journal › Article › peer-review

40 Citations (Scopus)

Abstract

Poly(hydroxyethyl methacrylate-ethylen glycol dimethacrylate), p(HEMA-EGDMA), beads were prepared by suspension polymerization, and were decorated with fibrous poly(glycidyl methacrylate), p(GMA), via surface initiated-atom transfer radical polymerization (SI-ATRP). The functional epoxy groups of the beads were used for covalent immobilization of papain. The average amount of immobilized enzyme was 18.7mg/g beads. The immobilized enzyme was characterized by temperature, pH, operational and storage stability experiments. The maximum velocity of the free and immobilized enzymes (V _max) and Michaelis-Menten constant (K _m) values were determined as 10.7 and 8.3U/mg proteins and 274 and 465μM, respectively. The immobilized papain was operated in a batch reactor, and it was very effective for hydrolysis of different proteins (i.e., casein and cytochrom c).

Original language	English
Pages (from-to)	9833-9837
Number of pages	5
Journal	Bioresource Technology
Volume	102
Issue number	21
DOIs	https://doi.org/10.1016/j.biortech.2011.08.042
Publication status	Published - Nov 2011

Keywords

Beads
Immobilized enzyme
Papain
Protein hydrolysis
SI-ATRP

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Bayramoglu, G., Filiz Senkal, B., Yilmaz, M., & Yakup Arica, M. (2011). Immobilization and stabilization of papain on poly(hydroxyethyl methacrylate-ethylenglycol dimethacrylate) beads grafted with epoxy functional polymer chains via surface-initiated-atom transfer radical polymerization (SI-ATRP). Bioresource Technology, 102(21), 9833-9837. https://doi.org/10.1016/j.biortech.2011.08.042

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title = "Immobilization and stabilization of papain on poly(hydroxyethyl methacrylate-ethylenglycol dimethacrylate) beads grafted with epoxy functional polymer chains via surface-initiated-atom transfer radical polymerization (SI-ATRP)",

abstract = "Poly(hydroxyethyl methacrylate-ethylen glycol dimethacrylate), p(HEMA-EGDMA), beads were prepared by suspension polymerization, and were decorated with fibrous poly(glycidyl methacrylate), p(GMA), via surface initiated-atom transfer radical polymerization (SI-ATRP). The functional epoxy groups of the beads were used for covalent immobilization of papain. The average amount of immobilized enzyme was 18.7mg/g beads. The immobilized enzyme was characterized by temperature, pH, operational and storage stability experiments. The maximum velocity of the free and immobilized enzymes (V max) and Michaelis-Menten constant (K m) values were determined as 10.7 and 8.3U/mg proteins and 274 and 465μM, respectively. The immobilized papain was operated in a batch reactor, and it was very effective for hydrolysis of different proteins (i.e., casein and cytochrom c).",

keywords = "Beads, Immobilized enzyme, Papain, Protein hydrolysis, SI-ATRP",

author = "Gulay Bayramoglu and {Filiz Senkal}, B. and Meltem Yilmaz and {Yakup Arica}, M.",

year = "2011",

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Bayramoglu, G, Filiz Senkal, B, Yilmaz, M & Yakup Arica, M 2011, 'Immobilization and stabilization of papain on poly(hydroxyethyl methacrylate-ethylenglycol dimethacrylate) beads grafted with epoxy functional polymer chains via surface-initiated-atom transfer radical polymerization (SI-ATRP)', Bioresource Technology, vol. 102, no. 21, pp. 9833-9837. https://doi.org/10.1016/j.biortech.2011.08.042

Immobilization and stabilization of papain on poly(hydroxyethyl methacrylate-ethylenglycol dimethacrylate) beads grafted with epoxy functional polymer chains via surface-initiated-atom transfer radical polymerization (SI-ATRP). / Bayramoglu, Gulay; Filiz Senkal, B.; Yilmaz, Meltem et al.
In: Bioresource Technology, Vol. 102, No. 21, 11.2011, p. 9833-9837.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Immobilization and stabilization of papain on poly(hydroxyethyl methacrylate-ethylenglycol dimethacrylate) beads grafted with epoxy functional polymer chains via surface-initiated-atom transfer radical polymerization (SI-ATRP)

AU - Bayramoglu, Gulay

AU - Filiz Senkal, B.

AU - Yilmaz, Meltem

AU - Yakup Arica, M.

PY - 2011/11

Y1 - 2011/11

N2 - Poly(hydroxyethyl methacrylate-ethylen glycol dimethacrylate), p(HEMA-EGDMA), beads were prepared by suspension polymerization, and were decorated with fibrous poly(glycidyl methacrylate), p(GMA), via surface initiated-atom transfer radical polymerization (SI-ATRP). The functional epoxy groups of the beads were used for covalent immobilization of papain. The average amount of immobilized enzyme was 18.7mg/g beads. The immobilized enzyme was characterized by temperature, pH, operational and storage stability experiments. The maximum velocity of the free and immobilized enzymes (V max) and Michaelis-Menten constant (K m) values were determined as 10.7 and 8.3U/mg proteins and 274 and 465μM, respectively. The immobilized papain was operated in a batch reactor, and it was very effective for hydrolysis of different proteins (i.e., casein and cytochrom c).

AB - Poly(hydroxyethyl methacrylate-ethylen glycol dimethacrylate), p(HEMA-EGDMA), beads were prepared by suspension polymerization, and were decorated with fibrous poly(glycidyl methacrylate), p(GMA), via surface initiated-atom transfer radical polymerization (SI-ATRP). The functional epoxy groups of the beads were used for covalent immobilization of papain. The average amount of immobilized enzyme was 18.7mg/g beads. The immobilized enzyme was characterized by temperature, pH, operational and storage stability experiments. The maximum velocity of the free and immobilized enzymes (V max) and Michaelis-Menten constant (K m) values were determined as 10.7 and 8.3U/mg proteins and 274 and 465μM, respectively. The immobilized papain was operated in a batch reactor, and it was very effective for hydrolysis of different proteins (i.e., casein and cytochrom c).

KW - Beads

KW - Immobilized enzyme

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Bayramoglu G, Filiz Senkal B, Yilmaz M, Yakup Arica M. Immobilization and stabilization of papain on poly(hydroxyethyl methacrylate-ethylenglycol dimethacrylate) beads grafted with epoxy functional polymer chains via surface-initiated-atom transfer radical polymerization (SI-ATRP). Bioresource Technology. 2011 Nov;102(21):9833-9837. doi: 10.1016/j.biortech.2011.08.042

Immobilization and stabilization of papain on poly(hydroxyethyl methacrylate-ethylenglycol dimethacrylate) beads grafted with epoxy functional polymer chains via surface-initiated-atom transfer radical polymerization (SI-ATRP)

Abstract

Keywords

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