Glycidyl methacrylate grafted on p(VBC) beads by SI-ATRP technique: Modified with hydrazine as a salt resistance ligand for adsorption of invertase

The cross-linked p(vinyl benzyl chloride), p(VBC), beads were prepared by suspension polymerization, and p(glycidylmethacrylate), p(GMA), was grafted on the surface of the beads using surface-initiated atom transfer radical polymerization, (SI-ATRP). The epoxy groups of the p(GMA) were reacted with a salt resistance ligand (i.e., hydrazine). The hydrazine-functionalized beads were used for adsorption of invertase. The influence of pH, equilibrium time, ionic strength, temperature and initial invertase concentration on the adsorption capacity of the affinity beads has been investigated. Maximum invertase adsorption onto affinity beads was 86.7 mg/g at pH 4.0. The experimental equilibrium data fitted well to the Dubinin-Radushkevich isotherm models.