Glucose sensitivity of polypyrrole-glucose oxidase modified electrodes containing p-benzoquinone in biological buffer

Ipek Becerik*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

The entrapping of enzymes in a polypyrrole (PPy) matrix during electropolimerization is attacting great interest as an immobilization technique, because it is simple and speady for the production of biosensors. PPy/Glucose oxidase (GOD) modified electrodes, which catalyse the oxidation of glucose to gluconolactone are prepared by the electropolimerization of pyrrole from a solution containing GOD in the presence of p.benzoquinone (Q).Q acts as a mediator for the electron transfer between GOD and the electrode. The phosphate buffer which is generally used is very nucleophilic and prevents a homogeneous growth of PPy films, therefore, it has been replaced by PIPES buffer (pka=6.8). GOD and Q are incorporated into polypyrrole films and provide electrical communications. Glucose sensitivity depends on the deposition charge of polymer films and there is an optimum deposition charge. Moreover, in this study, the response curves were kinetically analyzed and apparent Michaelis constants (Km) and apparent maximum rate(Imax) were determined.

Original languageEnglish
Pages (from-to)697-704
Number of pages8
JournalAnnali di Chimica
Volume88
Issue number9-10
Publication statusPublished - Sept 1998

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