TY - JOUR
T1 - Functionalized bicomponent polymer membranes as supports for covalent immobilization of enzymes
AU - Sandu, Teodor
AU - Sarbu, Andrei
AU - Damian, Celina Maria
AU - Patroi, Delia
AU - Iordache, Tanta Verona
AU - Budinova, Temenuzhka
AU - Tsyntsarski, Boyko
AU - Ferhat Yardim, M.
AU - Sirkecioglu, Ahmet
N1 - Publisher Copyright:
© 2015 Elsevier B.V. All rights reserved.
PY - 2015/11/1
Y1 - 2015/11/1
N2 - The present work was aimed at developing new reactive polymer materials suitable to be used as supports for enzyme covalent immobilization. Thus, new bicomponent polymer membranes were developed using poly(acrylonitrile-co-vinyl acetate) (PAN-co-PVAc) in mixture with polyvinyl alcohol (PVA). First of all, PAN-co-PVAc/PVA blends were dissolved in DMSO, until a homogenous polymer solution was obtained. To prepare membranes, these solutions were cast on a glass plate followed by the immersion of this plate in a coagulation bath containing a 50%-50% (volume %) water-isopropyl alcohol mixture. In this way membranes containing OH functional groups were obtained. Before tyrosinase immobilization, membranes were functionalized with glutardialdehyde; hence CHO binding sites were inserted and membrane became reactive for the enzyme. In order to prove that both, functionalization and immobilization reactions, were successful, the modifications produced by these reactions were investigated by various techniques i.e. Fourier Transform Infrared Spectrometry, Thermal Gravimetric Analysis, Differential Scanning Calorimetry and Atomic Force Microscopy. The occurrence of important changes in membrane features confirmed the success of both reactions. Furthermore, the activity of bonded enzyme was determined by pyrocatechol method and compared to the activity of the free enzyme.
AB - The present work was aimed at developing new reactive polymer materials suitable to be used as supports for enzyme covalent immobilization. Thus, new bicomponent polymer membranes were developed using poly(acrylonitrile-co-vinyl acetate) (PAN-co-PVAc) in mixture with polyvinyl alcohol (PVA). First of all, PAN-co-PVAc/PVA blends were dissolved in DMSO, until a homogenous polymer solution was obtained. To prepare membranes, these solutions were cast on a glass plate followed by the immersion of this plate in a coagulation bath containing a 50%-50% (volume %) water-isopropyl alcohol mixture. In this way membranes containing OH functional groups were obtained. Before tyrosinase immobilization, membranes were functionalized with glutardialdehyde; hence CHO binding sites were inserted and membrane became reactive for the enzyme. In order to prove that both, functionalization and immobilization reactions, were successful, the modifications produced by these reactions were investigated by various techniques i.e. Fourier Transform Infrared Spectrometry, Thermal Gravimetric Analysis, Differential Scanning Calorimetry and Atomic Force Microscopy. The occurrence of important changes in membrane features confirmed the success of both reactions. Furthermore, the activity of bonded enzyme was determined by pyrocatechol method and compared to the activity of the free enzyme.
KW - Acrylonitrile copolymer
KW - Enzymes immobilization
KW - Glutardialdehyde functionalization
KW - Polyvinyl alcohol
KW - Tyrosinase
UR - http://www.scopus.com/inward/record.url?scp=84941621134&partnerID=8YFLogxK
U2 - 10.1016/j.reactfunctpolym.2015.09.001
DO - 10.1016/j.reactfunctpolym.2015.09.001
M3 - Article
AN - SCOPUS:84941621134
SN - 1381-5148
VL - 96
SP - 5
EP - 13
JO - Reactive and Functional Polymers
JF - Reactive and Functional Polymers
ER -