Abstract
Due to their structural characteristics at the nanoscale level, single-walled carbon nanotubes (SWNTs), hold great promise for applications in biomedicine such as drug delivery systems. Herein, a novel single-walled carbon nanotube (SWNT)-based drug delivery system was developed by conjugation of various Fmoc-amino acid bearing polyethylene glycol (PEG) chains (Mw = 2,000, 5,000, and 12,000). In the first step, full-atom molecular dynamics simulations (MD) were performed to identify the most suitable Fmoc-amino acid for an effective surface coating of SWNT. Fmoc-glycine, Fmoc-tryptophan, and Fmoc-cysteine were selected to attach to the PEG polymer. Here, Fmoc-cysteine and -tryptophan had better average interaction energies with SWNT with a high number of aromatic groups, while Fmoc-glycine provided a non-aromatic control. In the experimental studies, non-covalent modification of SWNTs was achieved by Fmoc-amino acid-bearing PEG chains. The remarkably high amount of Fmoc-glycine-PEG, Fmoc-tryptophan-PEG, and Fmoc-cysteine-PEG complexes adsorbed onto the SWNT surface, as was assessed via thermogravimetric and UV-vis spectroscopy analyses. Furthermore, Fmoc-cysteine-PEG5000 and Fmoc-cysteine-PEG12000 complexes displayed longer suspension time in deionized water, up to 1 and 5 week, respectively, underlying the ability of these surfactants to effectively disperse SWNTs in an aqueous environment. In vitro cell viability assays on human dermal fibroblast cells also showed the low cytotoxicity of these two samples, even at high concentrations. In conclusion, synthesized nanocarriers have a great potential for drug delivery systems, with high loading capacity, and excellent complex stability in water critical for biocompatibility.
Original language | English |
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Article number | 648366 |
Journal | Frontiers in Bioengineering and Biotechnology |
Volume | 9 |
DOIs | |
Publication status | Published - 14 May 2021 |
Bibliographical note
Publisher Copyright:© Copyright © 2021 Yeniyurt, Kilic, Güner-Yılmaz, Bozoglu, Meran, Baysak, Kurkcuoglu, Hizal, Karatepe, Batirel and Güner.
Funding
This work was funded by the Istanbul Technical University Scientific Research Projects Foundation, Project No. MGA-2019-41823.
Funders | Funder number |
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Istanbul Technical University Scientific Research Projects Foundation | MGA-2019-41823 |
Keywords
- Fmoc-protected amino acids
- PEG
- carbon nanotubes
- molecular dynamic simulation
- nanocarrier (nanoparticle)
- non-covalent modification