Existence of SARS-CoV-2 RNA on ambient particulate matter samples: A nationwide study in Turkey

Özgecan Kayalar, Akif Arı, Gizem Babuççu, Nur Konyalılar, Özlem Doğan, Füsun Can, Ülkü A. Şahin, Eftade O. Gaga, S. Levent Kuzu, Pelin Ertürk Arı, Mustafa Odabaşı, Yücel Taşdemir, S. Sıddık Cindoruk, Fatma Esen, Egemen Sakın, Burak Çalışkan, Lokman H. Tecer, Merve Fıçıcı, Ahmet Altın, Burcu OnatCoşkun Ayvaz, Burcu Uzun, Arslan Saral, Tuncay Döğeroğlu, Semra Malkoç, Özlem Özden Üzmez, Fatma Kunt, Senar Aydın, Melik Kara, Barış Yaman, Güray Doğan, Bihter Olgun, Ebru N. Dokumacı, Gülen Güllü, Elif S. Uzunpınar, Hasan Bayram*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

37 Citations (Scopus)

Abstract

Coronavirus disease 2019 (COVID-19) is caused by the SARS-CoV-2 virus and has been affecting the world since the end of 2019. The disease led to significant mortality and morbidity in Turkey, since the first case was reported on March 11th, 2020. Studies suggest a positive association between air pollution and SARS-CoV-2 infection. The aim of the present study was to investigate the role of ambient particulate matters (PM), as potential carriers for SARS-CoV-2. Ambient PM samples in various size ranges were collected from 13 sites including urban and urban-background locations and hospital gardens in 10 cities across Turkey between 13th of May and 14th of June 2020 to investigate the possible presence of SARS-CoV-2 RNA on ambient PM. A total of 203 daily samples (TSP, n = 80; PM2.5, n = 33; PM2.510, n = 23; PM10μm, n = 19; and 6 size segregated PM, n = 48) were collected using various samplers. The N1 gene and RdRP gene expressions were analyzed for the presence of SARS-CoV-2, as suggested by the Centers for Disease Control and Prevention (CDC). According to real time (RT)-PCR and three-dimensional (3D) digital (d) PCR analysis, dual RdRP and N1 gene positivity were detected in 20 (9.8%) samples. Ambient PM-bound SARS-CoV-2 was analyzed quantitatively and the air concentrations of the virus ranged from 0.1 copies/m3 to 23 copies/m3. The highest percentages of virus detection on PM samples were from hospital gardens in Tekirdağ, Zonguldak, and Istanbul, especially in PM2.5 mode. Findings of this study have suggested that SARS-CoV-2 may be transported by ambient particles, especially at sites close to the infection hot-spots. However, whether this has an impact on the spread of the virus infection remains to be determined.

Original languageEnglish
Article number147976
JournalScience of the Total Environment
Volume789
DOIs
Publication statusPublished - 1 Oct 2021
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2021 Elsevier B.V.

Keywords

  • 3D-dPCR
  • Airborne
  • Particulate matter
  • SARS-CoV-2

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