Evolutionary engineering of yeast

Ceren Alkım; Burcu Turanlı-yıldız; Z. Petek Çakar

doi:10.1007/978-1-4939-0563-8_10

Evolutionary engineering of yeast

Ceren Alkım, Burcu Turanlı-yıldız, Z. Petek Çakar

Department of Molecular Biology and Genetics

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Evolutionary engineering is an inverse metabolic engineering strategy which is based on increasing genetic diversity and screening large populations for desired phenotypes. This strategy is highly advantageous in certain situations over rational metabolic engineering approaches, since there is little or no requirement of detailed genetic background information for the trait of interest. Here, we describe the experimental methodology for selecting stress-resistant yeast strains via evolutionary engineering approach by either serial batch or chemostat cultivations.

Original language	English
Pages (from-to)	169-183
Number of pages	15
Journal	Methods in Molecular Biology
Volume	1152
DOIs	https://doi.org/10.1007/978-1-4939-0563-8_10
Publication status	Published - 2014

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media, LLC 2014.

Keywords

Batch selection
Chemostat selection
Evolutionary engineering
Inverse metabolic engineering

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10.1007/978-1-4939-0563-8_10

Cite this

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AU - Çakar, Z. Petek

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KW - Chemostat selection

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Evolutionary engineering of yeast

Abstract

Bibliographical note

Keywords

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