Evaluation of performance, acetoclastic methanogenic activity and archaeal composition of full-scale UASB reactors treating alcohol distillery wastewaters

Alper T. Akarsubasi; Orhan Ince; Nilgun A. Oz; Betul Kirdar; Bahar K. Ince

doi:10.1016/j.procbio.2005.01.029

Evaluation of performance, acetoclastic methanogenic activity and archaeal composition of full-scale UASB reactors treating alcohol distillery wastewaters

Alper T. Akarsubasi
, Orhan Ince
, Nilgun A. Oz
, Betul Kirdar
, Bahar K. Ince^*

^*Corresponding author for this work

Department of Environmental Engineering

Bogazici University

Research output: Contribution to journal › Article › peer-review

50 Citations (Scopus)

Abstract

Two distinct full-scale upflow anaerobic sludge blanket (UASB) reactors, namely IUASB (143 m³) and TUASB (476 m³), treating alcohol (raki) distillery wastewaters were investigated in terms of performance, acetoclastic methanogenic activity and archaeal composition. The TUASB reactor was inoculated with the seed sludge taken from the IUASB reactor. An average of 90% COD removal efficiency at organic loading rates (OLRs) in a range of 6-11 kg COD/m³ day was achieved in the IUASB reactor. However, 60-80% COD removal efficiency of the TUASB reactor was achieved at OLRs ranging 2.5-8.5 kg COD/m³ day. Both UASB reactors showed almost the same acetoclastic methanogenic activity (350 and 376 ml CH₄/g VSS day). Distribution of archaeal populations within the two UASB reactors was investigated by denaturing gradient gel electrophoresis analysis of PCR amplified ribosomal RNA gene fragments. Both UASB reactors have shown quite similar archaeal composition (weighted similarity of 83.1%). Predominant archaeal sequences in both reactors belonged to Methanobacterium formicicum and Methanosaeta soehngenii.

Original language	English
Pages (from-to)	28-35
Number of pages	8
Journal	Process Biochemistry
Volume	41
Issue number	1
DOIs	https://doi.org/10.1016/j.procbio.2005.01.029
Publication status	Published - Jan 2006

Funding

Author Alper T. Akarsubasi would like to acknowledge TUBITAK NATO-A2 fund and also Dr. Fahir Ilter Scholarship of Bogazici University Foundation for support to complete this study. Authors would also like to acknowledge the project coded 844, Research Fund of Istanbul Technical University, the project coded 01M102, Research Fund of Bogazici University and the project coded TBAG-1935 (100T054) of TUBITAK. Authors wish to thank particularly Dr. Tom Curtis, Dr. Ian Head and the Staff of University of Newcastle Upon Tyne, Department of Civil Engineering, UK and also Tekel Co. (Turkey) for their cooperation and supplying samples and some performance data of their treatment plants.

Funders	Funder number
Bogazici University	TBAG-1935, 100T054
Bogazici University Foundation	844
TUBITAK
TUBITAK NATO-A2 fund
Istanbul Teknik Üniversitesi	01M102

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 6 Clean Water and Sanitation

Keywords

16S rDNA analysis
Acetoclastic methanogenic activity
Alcohol distillery wastewater
Archaea
DGGE
UASB reactors

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10.1016/j.procbio.2005.01.029

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title = "Evaluation of performance, acetoclastic methanogenic activity and archaeal composition of full-scale UASB reactors treating alcohol distillery wastewaters",

abstract = "Two distinct full-scale upflow anaerobic sludge blanket (UASB) reactors, namely IUASB (143 m3) and TUASB (476 m3), treating alcohol (raki) distillery wastewaters were investigated in terms of performance, acetoclastic methanogenic activity and archaeal composition. The TUASB reactor was inoculated with the seed sludge taken from the IUASB reactor. An average of 90\% COD removal efficiency at organic loading rates (OLRs) in a range of 6-11 kg COD/m3 day was achieved in the IUASB reactor. However, 60-80\% COD removal efficiency of the TUASB reactor was achieved at OLRs ranging 2.5-8.5 kg COD/m3 day. Both UASB reactors showed almost the same acetoclastic methanogenic activity (350 and 376 ml CH4/g VSS day). Distribution of archaeal populations within the two UASB reactors was investigated by denaturing gradient gel electrophoresis analysis of PCR amplified ribosomal RNA gene fragments. Both UASB reactors have shown quite similar archaeal composition (weighted similarity of 83.1\%). Predominant archaeal sequences in both reactors belonged to Methanobacterium formicicum and Methanosaeta soehngenii.",

keywords = "16S rDNA analysis, Acetoclastic methanogenic activity, Alcohol distillery wastewater, Archaea, DGGE, UASB reactors",

author = "Akarsubasi, \{Alper T.\} and Orhan Ince and Oz, \{Nilgun A.\} and Betul Kirdar and Ince, \{Bahar K.\}",

year = "2006",

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doi = "10.1016/j.procbio.2005.01.029",

language = "English",

volume = "41",

pages = "28--35",

journal = "Process Biochemistry",

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AU - Akarsubasi, Alper T.

AU - Ince, Orhan

AU - Oz, Nilgun A.

AU - Kirdar, Betul

AU - Ince, Bahar K.

PY - 2006/1

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N2 - Two distinct full-scale upflow anaerobic sludge blanket (UASB) reactors, namely IUASB (143 m3) and TUASB (476 m3), treating alcohol (raki) distillery wastewaters were investigated in terms of performance, acetoclastic methanogenic activity and archaeal composition. The TUASB reactor was inoculated with the seed sludge taken from the IUASB reactor. An average of 90% COD removal efficiency at organic loading rates (OLRs) in a range of 6-11 kg COD/m3 day was achieved in the IUASB reactor. However, 60-80% COD removal efficiency of the TUASB reactor was achieved at OLRs ranging 2.5-8.5 kg COD/m3 day. Both UASB reactors showed almost the same acetoclastic methanogenic activity (350 and 376 ml CH4/g VSS day). Distribution of archaeal populations within the two UASB reactors was investigated by denaturing gradient gel electrophoresis analysis of PCR amplified ribosomal RNA gene fragments. Both UASB reactors have shown quite similar archaeal composition (weighted similarity of 83.1%). Predominant archaeal sequences in both reactors belonged to Methanobacterium formicicum and Methanosaeta soehngenii.

AB - Two distinct full-scale upflow anaerobic sludge blanket (UASB) reactors, namely IUASB (143 m3) and TUASB (476 m3), treating alcohol (raki) distillery wastewaters were investigated in terms of performance, acetoclastic methanogenic activity and archaeal composition. The TUASB reactor was inoculated with the seed sludge taken from the IUASB reactor. An average of 90% COD removal efficiency at organic loading rates (OLRs) in a range of 6-11 kg COD/m3 day was achieved in the IUASB reactor. However, 60-80% COD removal efficiency of the TUASB reactor was achieved at OLRs ranging 2.5-8.5 kg COD/m3 day. Both UASB reactors showed almost the same acetoclastic methanogenic activity (350 and 376 ml CH4/g VSS day). Distribution of archaeal populations within the two UASB reactors was investigated by denaturing gradient gel electrophoresis analysis of PCR amplified ribosomal RNA gene fragments. Both UASB reactors have shown quite similar archaeal composition (weighted similarity of 83.1%). Predominant archaeal sequences in both reactors belonged to Methanobacterium formicicum and Methanosaeta soehngenii.

KW - 16S rDNA analysis

KW - Acetoclastic methanogenic activity

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KW - Archaea

KW - DGGE

KW - UASB reactors

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Evaluation of performance, acetoclastic methanogenic activity and archaeal composition of full-scale UASB reactors treating alcohol distillery wastewaters

Abstract

Funding

UN SDGs

Keywords

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