Evaluating bioactivity and bioaccessibility properties of the propolis extract prepared with L-lactic acid: An alternative solvent to ethanol for propolis extraction

Ali Timucin Atayoglu; Didem Sözeri Atik; Esra Bölük; Başak Gürbüz; Fatma Duygu Ceylan; Esra Çapanoğlu; Rumeysa Atayolu; Anant Paradkar; James Fearnley; Ibrahim Palabiyik

doi:10.1016/j.fbio.2023.102756

Evaluating bioactivity and bioaccessibility properties of the propolis extract prepared with L-lactic acid: An alternative solvent to ethanol for propolis extraction

Ali Timucin Atayoglu, Didem Sözeri Atik^*, Esra Bölük, Başak Gürbüz, Fatma Duygu Ceylan, Esra Çapanoğlu, Rumeysa Atayolu, Anant Paradkar, James Fearnley, Ibrahim Palabiyik

^*Corresponding author for this work

Department of Food Engineering

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

Ethanol readily solubilizes the active components of propolis. Due to certain disadvantages of ethanolic extracts, developing new non-ethanolic extraction methods is highly desirable. This study aimed to determine the content of phenolic compounds and antioxidant activity of propolis by using lactic acid extraction compared to ethanol. Various concentrations of ethanol and lactic acid were utilized to produce extracts, and an in vitro digestion procedure was applied to the extracts to compare the bioaccessibility of phenolic compounds by using LC-MS/MS in extracts and HPLC-DAD in digested samples. The compounds detected in the lactic acid-based propolis solution had similar values to those in the ethanol solution at a propolis concentration of 10%. The propolis-lactic acid solution had a significantly higher antioxidant activity against DPPH radical than the one prepared with ethanol at the same concentration (p < 0.05). Chrysin and naringenin were found as the most abundant phenolic compounds in the initial samples during in vitro digestion experiments. Pinocembrin was the most bioaccessible component among the dialyzed IN fractions of ethanol and lactic acid-based solutions, whereas some compounds were not detected. The results suggest that lactic acid may be used as an alternative to ethanol for propolis extraction.

Original language	English
Article number	102756
Journal	Food Bioscience
Volume	53
DOIs	https://doi.org/10.1016/j.fbio.2023.102756
Publication status	Published - Jun 2023

Bibliographical note

Publisher Copyright:
© 2023 Elsevier Ltd

Keywords

Antioxidant
Extraction
Lactic acid
Phenolics
Propolis

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10.1016/j.fbio.2023.102756

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Atayoglu, A. T., Sözeri Atik, D., Bölük, E., Gürbüz, B., Ceylan, F. D., Çapanoğlu, E., Atayolu, R., Paradkar, A., Fearnley, J., & Palabiyik, I. (2023). Evaluating bioactivity and bioaccessibility properties of the propolis extract prepared with L-lactic acid: An alternative solvent to ethanol for propolis extraction. Food Bioscience, 53, Article 102756. https://doi.org/10.1016/j.fbio.2023.102756

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title = "Evaluating bioactivity and bioaccessibility properties of the propolis extract prepared with L-lactic acid: An alternative solvent to ethanol for propolis extraction",

abstract = "Ethanol readily solubilizes the active components of propolis. Due to certain disadvantages of ethanolic extracts, developing new non-ethanolic extraction methods is highly desirable. This study aimed to determine the content of phenolic compounds and antioxidant activity of propolis by using lactic acid extraction compared to ethanol. Various concentrations of ethanol and lactic acid were utilized to produce extracts, and an in vitro digestion procedure was applied to the extracts to compare the bioaccessibility of phenolic compounds by using LC-MS/MS in extracts and HPLC-DAD in digested samples. The compounds detected in the lactic acid-based propolis solution had similar values to those in the ethanol solution at a propolis concentration of 10%. The propolis-lactic acid solution had a significantly higher antioxidant activity against DPPH radical than the one prepared with ethanol at the same concentration (p < 0.05). Chrysin and naringenin were found as the most abundant phenolic compounds in the initial samples during in vitro digestion experiments. Pinocembrin was the most bioaccessible component among the dialyzed IN fractions of ethanol and lactic acid-based solutions, whereas some compounds were not detected. The results suggest that lactic acid may be used as an alternative to ethanol for propolis extraction.",

keywords = "Antioxidant, Extraction, Lactic acid, Phenolics, Propolis",

author = "Atayoglu, {Ali Timucin} and {S{\"o}zeri Atik}, Didem and Esra B{\"o}l{\"u}k and Ba{\c s}ak G{\"u}rb{\"u}z and Ceylan, {Fatma Duygu} and Esra {\c C}apanoğlu and Rumeysa Atayolu and Anant Paradkar and James Fearnley and Ibrahim Palabiyik",

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year = "2023",

month = jun,

doi = "10.1016/j.fbio.2023.102756",

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volume = "53",

journal = "Food Bioscience",

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Atayoglu, AT, Sözeri Atik, D, Bölük, E, Gürbüz, B, Ceylan, FD, Çapanoğlu, E, Atayolu, R, Paradkar, A, Fearnley, J & Palabiyik, I 2023, 'Evaluating bioactivity and bioaccessibility properties of the propolis extract prepared with L-lactic acid: An alternative solvent to ethanol for propolis extraction', Food Bioscience, vol. 53, 102756. https://doi.org/10.1016/j.fbio.2023.102756

TY - JOUR

T1 - Evaluating bioactivity and bioaccessibility properties of the propolis extract prepared with L-lactic acid

T2 - An alternative solvent to ethanol for propolis extraction

AU - Atayoglu, Ali Timucin

AU - Sözeri Atik, Didem

AU - Bölük, Esra

AU - Gürbüz, Başak

AU - Ceylan, Fatma Duygu

AU - Çapanoğlu, Esra

AU - Atayolu, Rumeysa

AU - Paradkar, Anant

AU - Fearnley, James

AU - Palabiyik, Ibrahim

PY - 2023/6

Y1 - 2023/6

N2 - Ethanol readily solubilizes the active components of propolis. Due to certain disadvantages of ethanolic extracts, developing new non-ethanolic extraction methods is highly desirable. This study aimed to determine the content of phenolic compounds and antioxidant activity of propolis by using lactic acid extraction compared to ethanol. Various concentrations of ethanol and lactic acid were utilized to produce extracts, and an in vitro digestion procedure was applied to the extracts to compare the bioaccessibility of phenolic compounds by using LC-MS/MS in extracts and HPLC-DAD in digested samples. The compounds detected in the lactic acid-based propolis solution had similar values to those in the ethanol solution at a propolis concentration of 10%. The propolis-lactic acid solution had a significantly higher antioxidant activity against DPPH radical than the one prepared with ethanol at the same concentration (p < 0.05). Chrysin and naringenin were found as the most abundant phenolic compounds in the initial samples during in vitro digestion experiments. Pinocembrin was the most bioaccessible component among the dialyzed IN fractions of ethanol and lactic acid-based solutions, whereas some compounds were not detected. The results suggest that lactic acid may be used as an alternative to ethanol for propolis extraction.

AB - Ethanol readily solubilizes the active components of propolis. Due to certain disadvantages of ethanolic extracts, developing new non-ethanolic extraction methods is highly desirable. This study aimed to determine the content of phenolic compounds and antioxidant activity of propolis by using lactic acid extraction compared to ethanol. Various concentrations of ethanol and lactic acid were utilized to produce extracts, and an in vitro digestion procedure was applied to the extracts to compare the bioaccessibility of phenolic compounds by using LC-MS/MS in extracts and HPLC-DAD in digested samples. The compounds detected in the lactic acid-based propolis solution had similar values to those in the ethanol solution at a propolis concentration of 10%. The propolis-lactic acid solution had a significantly higher antioxidant activity against DPPH radical than the one prepared with ethanol at the same concentration (p < 0.05). Chrysin and naringenin were found as the most abundant phenolic compounds in the initial samples during in vitro digestion experiments. Pinocembrin was the most bioaccessible component among the dialyzed IN fractions of ethanol and lactic acid-based solutions, whereas some compounds were not detected. The results suggest that lactic acid may be used as an alternative to ethanol for propolis extraction.

KW - Antioxidant

KW - Extraction

KW - Lactic acid

KW - Phenolics

KW - Propolis

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U2 - 10.1016/j.fbio.2023.102756

DO - 10.1016/j.fbio.2023.102756

M3 - Article

AN - SCOPUS:85160066079

SN - 2212-4292

VL - 53

JO - Food Bioscience

JF - Food Bioscience

M1 - 102756

ER -

Evaluating bioactivity and bioaccessibility properties of the propolis extract prepared with L-lactic acid: An alternative solvent to ethanol for propolis extraction

Abstract

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Keywords

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